蜂胶对金黄色葡萄球菌α-溶血素的抑制作用  

Inhibition of propolis on the production of α-hemolysin by Staphylococcus aureus

作　　者：陈志宝[1] 姜游帅[1] 李欣燃[1] 李琳[1] 

机构地区：[1]黑龙江八一农垦大学生命科技学院,黑龙江大庆163319

出　　处：《中国兽医学报》2012年第8期1190-1193,共4页Chinese Journal of Veterinary Science

基　　金：教育部人兽共患病研究所开放基金资助项目(450060445319)

摘　　要：通过吸光度测量和荧光定量PCR的方法,在体外检测亚抑菌浓度的蜂胶作用下金黄色葡萄球菌α-溶血素活力变化及agrA、hla的mRNA表达量。结果显示,不同浓度蜂胶对金黄色葡萄球菌α-溶血素有不同程度的抑制作用(P<0.05);同时荧光定量PCR检测agrA和hla基因表明,蜂胶能够显著减少agrA和hla mRNA的相对表达量,进而抑制α-溶血素的表达。To study the inhibition of propolis on the production of a-hemolysin by Staphylococcus aureus, the experi- ment detected the changes of a-hemolysin and mRNA expression of agrA and hla under subinhibitory concentration of propolis with the measurement of ahsorbance and real-time quantitative PCR. The results showed that the differ- ent concentration of propolis can reduce the production of virulence factors a-toxin. Meanwhile the determination of real-time quantitative PCR showed that propolis can also reduce the production of agrA and hla mRNA, further in- hibit the expression of α-hemolysin.

关 键 词：蜂胶 亚抑菌浓度 金黄色葡萄球菌 α-溶血素 

分 类 号：S852.61[农业科学—基础兽医学]