鸭血红素加氧酶-1(HO-1)基因表达及对蛋壳颜色的影响  

Expression of heme oxygenase-1(HO-1) in different ducks and its affection of eggshell colors

作　　者：岳慧洁[1] 徐善金[1] 韦小菲[1] 赵文超[1] 虞德兵[1] 杜文兴[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《中国兽医学报》2012年第8期1235-1238,共4页Chinese Journal of Veterinary Science

基　　金：教育部高校博士点基金新教师基金资助项目(200803071048);南京农业大学青年科技创新基金资助项目(KJ09013);江苏省农业科技自主创新项目(cx(09)116);江苏省自然科学基金资助项目(SBK201021501)

摘　　要：以35周龄产绿壳蛋巢湖鸭、产白壳蛋巢湖鸭以及樱桃谷鸭为素材,采集每只鸭的肝脏和输卵管子宫部,提取总RNA进行cDNA反转录,利用实时荧光定量PCR(real-time PCR)方法检测样品中血红素加氧酶(heme oxygen-ase,HO-1)基因的表达水平,分析不同品种、品系间及同一品种不同器官中HO-1mRNA的表达差异。结果显示:不同品种间,HO-1基因在肝脏组织中相对表达量无显著差异(P>0.05),在子宫组织中表达量巢湖鸭极显著高于樱桃谷鸭(P<0.01)。不同品系间,HO-1基因在肝脏组织中相对表达量无显著性差异(P>0.05);巢湖鸭2个品系在子宫中的相对表达量差异不显著,但均显著高于樱桃谷鸭。在同一品种内,HO-1基因在肝脏组织中的相对表达量均极显著高于子宫组织(P<0.01)。Collect every duck liver and interstitial part of uterine tube from Cherry Valley,green eggshell Chaohu and white eggshell Chaohu 35-week-old,total RNA of liver and uterus was extracted and reversed transcription cDNA, the heme oxygenase （HO-1） mRNA expression level in different organs of different species were detected by using real-time PCR method. Results suggested that there is no significant difference in expression level of HO-1 of livers in different varieties（P〈0.05）, but highly significant difference between uterus of Chaohu and Cherry Valley（P〈 0. 01）,and no difference between white eggshell Chaohu and green eggshell Chaohu. For the same variety, the ex- pression level of HO-1 in livers is significantly higher than in uterus （P〈0.01）.

关 键 词：血红素加氧酶 鸭 蛋壳颜色 实时荧光定量PCR 

分 类 号：S834[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]