190例乙型肝炎病毒感染患者血清相关指标分析  

Infection-related indicators in serum of 190 patients diagnosed with hepatitis B virus

作　　者：张国祥[1] 许文龙[1] 王红旗[1] 全建军[1] 雷和月 叶君[1] 

机构地区：[1]浙江萧山医院检验科,浙江杭州311202

出　　处：《中华医院感染学杂志》2012年第16期3565-3567,共3页Chinese Journal of Nosocomiology

基　　金：杭州市萧山区科技局课题(2010702)

摘　　要：目的分析乙型肝炎病毒感染患者血清相关指标的临床意义。方法应用微粒子化学发光法(CMIA)与酶联免疫吸附试验(ELISA)检测乙型肝炎表面抗原(HBsAg),应用荧光定量PCR法(FQ-PCR)检测HBV-DNA,HBsAg中和确证试验应用CMIA法。结果 190份标本中,应用CMIA法HBsAg的阳性率为98.4%,ELISA方法阳性率为91.1%;190份标本经CMIA法中和确证试验阳性率为96.8%,其中3例中和确证试验无法确认,但HBV-DNA阳性,3例中和确证试验无法确认者,CMIA法HBsAg与HBV-DNA阴性且两周后复查结果一致。结论 ELISA方法在低浓度HBsAg的检测上与CMIA相比存在不足,对首次乙型肝炎HBsAg检测的标本应首选CMIA进行检测,对低浓度HBsAg标本应多指标联合检测与确认。OBJECTIVE To study the clinical significance of infection-related indicators in the serum of 190 patients diagnosed with hepatitis B virus. METHODS HBsAg was tested by chemiluminescent mieropaticle immunoassay （CMIA） and enzyme-linked immunosorbent assay （ELISA）, HBV-DNA was detected by fluorescence quantitative PCR （FQ-PCR）, HBsAg was confirmed by CMIA neutralization test. RESULTS Among 190 samples, there were 98.4% of the samples that were HBsAg positive by CMIA and 91.1% of the samples were HBsAg positive by ELISA, the positive rate of 190 samples was 96. 8% through CMIA neutralization test, 3 cases could not be confirmed, but HBV-DNA was tested positive; among 3 cases who could not be confirmed with neutralization test, both HBV-DNA and HBsAg were negative and the result was the same two weeks after the reexamination. CONCLUSION Compared with quantitative methods such as CMIA for the detection of the concentration of HBsAg, ELISA is obviously insufficient. The laboratory should prefer sensitive CMIA for the first-time detection of HBsAg and collect double serum sample for the low-concentration HBsAg-positive patients.

关 键 词：乙型肝炎表面抗原 微粒子化学发光免疫分析技术 抗体 中和确认试验 

分 类 号：R512.6[医药卫生—内科学]