脉络宁对老年性痴呆模型大鼠脑组织SIRT1基因表达的影响  

Influence of Mailuoning on SIRT1 expression of brain tissue in Alzheimer′s disease rat model

作　　者：程念[1] 胡进中[1] 田玲玲[1] 赵欣欣[1] 申军华[1] 李芳芳[1] 

机构地区：[1]河北医科大学生物化学和分子生物学教研室,河北石家庄050017

出　　处：《中国老年学杂志》2012年第16期3462-3464,共3页Chinese Journal of Gerontology

基　　金：2005年度河北省科学技术研究与发展指导计划支持项目(05276454)

摘　　要：目的观察中药复方脉络宁和谢海洲方,西药维生素(C+E)和阿托伐他汀药物干预对老年痴呆(AD)模型大鼠脑组织SIRT1基因表达的影响。方法用去卵巢同时腹腔注射D-半乳糖大鼠模拟早期AD模型,予以中药复方脉络宁和谢海洲方,西药维生素(C+E)和阿托伐他汀干预,采用Morris水迷宫检测大鼠用药前后学习记忆能力的变化,采用RT-PCR,Western印迹技术检测大鼠颞叶右侧皮层SIRT1基因表达的变化。结果与假手术组相比,去卵巢并注射D-半乳糖组逃避潜伏期明显延长;给药后,与病理组相比,四个给药组总逃避潜伏期明显缩短。与假手术组相比,病理组的SIRT1 mRNA和蛋白质的相对表达量明显降低,而与病理组相比,四个给药组的SIRT1 mRNA和蛋白质的相对表达量均有不同程度的升高。结论本模型大鼠颞叶皮层SIRT1 mRNA和蛋白质表达水平均降低;而脉络宁,谢海洲方,维生素(C+E),阿托伐他汀均可以不同程度的升高SIRT1 mRNA和蛋白质的表达水平,提示SIRT1表达的增加可能是这些药物对AD大鼠模型的神经保护作用分子机制之一。Objective To investigate the effect of Mailuoning,traditional Chinese medicine recipe（Xie haizhou）,vitamin C＋E and atorvastatin on the expression of SIRT1 gene.Methods Ovariectomized rats injected with D-gal were selected.Learning and memory abilities were evaluated by Morris water maze.Content of SIRT1 mRNA was measured by RT-PCR.Content of SIRT1 protein was measured by Western-blotting.Results Escape latency was longer in the OVX＋D-gal group compared with that of sham group,and shorter in treatment groups compared with that of the OVX＋D-gal group.Compared with those of sham,the expressions of SIRT1 mRNA and protein were lower in those of OVX＋D-gal group.Compared with OVX＋D-gal group,the expressions of SIRT1 mRNA and protein in treatments group were higher.Conclusions Mailuoning,traditional Chinese medicine recipe（Xie haizhou）,vitamins（C＋E） and atorvastatin can increase the expression of SIRT1 mRNA and protein levels respectively in different degrees,which suggests that increased expression of SIRT1 could be one of the molecular mechanisms of neuroprotection of these drugs on AD rat model.

关 键 词：阿尔茨海默病 去卵巢 D-半乳糖 SIRT1 脉络宁 

分 类 号：R339.38[医药卫生—人体生理学] R34[医药卫生—基础医学]