浅谈工程化软骨组织的体内外培养  

In vitro and in vivo culture of engineered cartilage

作　　者：李奎锋[1] 郭立坤[1] 郭筵[1] 

机构地区：[1]四川大学国家生物医学材料工程技术研究中心,四川成都610065

出　　处：《求医问药（下半月刊）》2012年第6期321-322,共2页Seek Medical and Ask The Medicine

摘　　要：培养环境对工程化组织的形成至关重要。本研究采用两种胶原水凝胶为支架,包载骨髓基质干细胞,构建工程化软骨组织,分别进行体外培养和兔关节腔植入培养,通过细胞活性观察和组织学染色,分析两种培养方式的效果。结果表明,动物体内具有比体外培养更好的生长环境,利于细胞增殖和分化。The culture environment is of great importance for engineered tissue formation.In this study,two kinds of collagen hydrogel scaffolds were adopted,encapsulating with bone marrow stromal stem cells to build engineered cartilage,and were cultured in vitro and implanted in rabbit articular cavity.Cell activity and histological staining were performed to analysis the difference.The results showed that the in vivo environment was better for cell proliferation and differentiation than in vitro culture.

关 键 词：胶原 水凝胶 组织工程 软骨 培养环境 

分 类 号：R318.08[医药卫生—生物医学工程]