The interaction of GSSG modified magnetic nanoparticles with SPC-A1 cells in vitro  

作　　者：MA YongJie YAN ZhuBing XU Hong GU HongChen 

机构地区：[1]Nano Biomedical Research Center,School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China

出　　处：《Chinese Science Bulletin》2012年第26期3525-3531,共7页

基　　金：supported by the International Cooperation Project (20080068and 075207012);the National Natural Science Foundation of China(81000656)

摘　　要：Magnetic nanoparticles(MNPs) are promising materials for various biomedical applications,including magnetic resonance imaging,stem cell tracking,gene/drug delivery,and cancer treatment.To increase the effectiveness of MNPs,high capture efficiency and controlled uptake of the particles by cells is required.In this paper we report the cytotoxicity and cellular uptake into SPC-A1 cells of oxidized glutathione(GSSG)-modified MNPs(GSSG@Fe3O4).Experimental findings indicated that GSSG@Fe3O4 were biocompatible,and could be efficiently taken up by SPC-A1 cells(up to 160 pg iron per cell).The internalized GSSG@Fe3O4 was retained in the cell cytoplasm for 6 generations.The uptake of GSSG@Fe3O4 into SPC-A1 cells was energy-,concentration-and time-dependent.Pinocytosis may be involved in the internalization process of GSSG@Fe3O4 into SPC-A1 cells,but this mechanism remains to be elucidated.The controlled and efficient localization of GSSG@Fe3O4 into the cytosol and long intracellular retention provides theoretical and experimental insight into the biomedical applications for these molecules.Magnetic nanoparticles （MNPs） are promising materials for various biomedical applications, including magnetic resonance imag- ing, stem cell tracking, gene/drug delivery, and cancer treatment. To increase the effectiveness of MNPs, high capture efficiency and controlled uptake of the particles by cells is required. In this paper we report the cytotoxicity and cellular uptake into SPC-A1 cells of oxidized glutathione （GSSG）-modified MNPs （GSSG@Fe304）. Experimental findings indicated that GSSG@Fe304 were biocompatible, and could be efficiently taken up by SPC-A1 cells （up to 160 pg iron per cell）. The internalized GSSG@Fe304 was retained in the cell cytoplasm for 6 generations. The uptake of GSSG@Fe304 into SPC-A1 cells was energy-, concentration- and time-dependent. Pinocytosis may be involved in the internalization process of GSSG@Fe304 into SPC-A1 cells, but this mecha- nism remains to be elucidated. The controlled and efficient localization of GSSG@Fe304 into the cytosol and long intracellular retention provides theoretical and experimental insight into the biomedical applications for these molecules.

关 键 词：磁性纳米粒子 GSSG 干细胞 相互作用 修饰 Fe304 生物医学应用 磁共振成像技术 

分 类 号：R318.08[医药卫生—生物医学工程]