A Strategy for Detecting Absorbed Bioactive Compounds for Quality Control in the Water Extract of Rhubarb by Ultra Performance Liquid Chromatography with Photodiode Array Detector  

作　　者：王杨 黄熙 梁清华 范荣 秦锋 呙勇 闫奎坡 刘维 罗杰坤 李云辉 毛喜莲 刘昭前 周宏灏 

机构地区：[1]Institute of Integrated Traditional Chinese and Western Medicine,and the National Key Clinical Specialist Vocational School of Traditional Chinese Medicine Encephalopathy,Xiangya Hospital,Central South University [2]Department of Integrated Traditional Chinese and Western Medicine,Xiangya Hospital,Central South University [3]Department of Neurosurgery,Xiangya Hospital,Central South University [4]Institute of Clinical Pharmacology,Central South University

出　　处：《Chinese Journal of Integrative Medicine》2012年第9期690-698,共9页中国结合医学杂志（英文版）

基　　金：Supported by the Fund for Key Labo ratory of Traditional Chinese Medicine Gan of State Administration of Traditional Chinese Medicine;the Major Research Plan of the National Natural Science Foundation of China(No.90409010);was partly supported by the Huge Project to Boost Chinese Drug Development(No.2009ZX09304-003)

摘　　要：Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.Objective： To detect absorbed bioactive compounds of the water extract whose pharmacodynamic effect was craniocerebral protection for quality control assessment. Methods： Anthraquinones in water extract of rhubarb （WER）, in cerebrospinal fluid （CSF） of patients with traumatic brain injury （TBI） and in ipsilateral cortex of TBI rats following oral WER were respectively explored by ultra performance liquid chromatography with photodiode array detector （UPLC-PDA） method developed in the present study. The effects of anthraquinones absorbed into injured cortex on superoxidase dismutase （SOD） activity in TBI rats were detected. The antioxidative anthraquinones absorbed into target organ were evaluated for quality control of WER. Results： Anthraquinones in WER were aloe-emodin, rhein, emodin, chrysophanol, and physcion. Only the last anthraquinone was found in CSF and in ipsilateral cortex under this chromatographic condition. Physcion increased SOD activity in TBI rats significantly. Conclusions： Physcion was the main active compound of rhubarb against craniocerebral injury via antioxidant pathway. According to our strategy, the exploration of physcion suggested the possibility of a novel quality control of WER in treating TBI injury.

关 键 词：quality control absorbed bioactive compounds RHUBARB ultra performance liquid chromatography craniocerebral injury ANTHRAQUINONES 

分 类 号：R284.1[医药卫生—中药学]