骆驼刺内生菌分离及代谢物活性成分分析  

Isolation of Endophytic Bacteria from Alhagi pseudalhagi Desv. and Analysis of Active Metabolites

作　　者：阿依乃再·孜亚克[1] 吾甫尔·米吉提[1] 阿布都卡地尔·阿布力孜[1] 阿力木江·阿合约力[1] 古丽祖热·佐努尼[1] 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《西北植物学报》2012年第7期1466-1473,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(31160015)

摘　　要：采用研磨法首次从新疆药用植物骆驼刺茎和叶等组织中分离得到20株内生菌并从中筛选出一株对作物致病菌的拮抗活性最强的内生菌XJAS-AB-11。通过形态学观察,生理生化特征以及16SrDNA序列分析最终将菌株XJAS-AB-11初步鉴定为枯草芽孢杆菌属(Bacillus sp.)。进一步对该拮抗菌株进行扩大培养,对其代谢产物进行初步研究,经硅胶柱色谱及TLC从具有抑菌作用的活性部位分离出活性单体C2和H2-2。紫外光谱、核磁共振氢谱及质谱分析证明化合物C2和H2-2均属于黄酮类化合物。研究表明,骆驼刺植物内生菌中蕴藏着较丰富的抗菌活性物质,是开发天然抗菌药物的潜在资源。Total 20 strains of endophytic bacteria were isolated from different parts of Alhagi pseudalhagi Desv. for the first time by grinding method and 1 strain XJAS-AB-11 was screened out from them with the stronggest antagonistic activity. According to the physiological and biochemical characteristics, 16S rDNA sequencing analysis, the XJAS-AB-11 was identified as Bacillus sp. The antagonistic strain was further am- plification cultured and its metabolites was studied initially with column chromatography on silica gel and TLC chromatography. The active monomers C2 and H2-2 were obtained from the antibacterial phase. UV- spectra,lH-NMR and MS results showed that compound C2 and H2-2 were flavoniods. The molecular for- mula of these compounds need to be further studied. Our study indicated that A. pseudalhagi Desv. endo- phytes have plenty of antimicrobial substances as a potential resources of devolopement of natural antimi- crobile drugs.

关 键 词：骆驼刺 内生菌 鉴定 代谢产物 

分 类 号：Q939.92[生物学—微生物学] Q501