10个山茶岛屿天然居群的遗传多样性分析  

Analysis on Genetic Diversity of Ten Insular Populations of Camellia japonica

作　　者：林立[1,2] 胡仲义[2] 李纪元[3] 祝志勇[2] 倪穗[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211 [2]宁波城市职业技术学院,浙江宁波315502 [3]中国林业科学院亚热带林业研究所,浙江富阳311400

出　　处：《园艺学报》2012年第8期1531-1538,共8页Acta Horticulturae Sinica

基　　金：浙江省科技厅重点项目(2008C14065);宁波市国际科技合作项目(2010D10013);宁波市农村科技创新创业资金项目(2010C91026);国家国际科技合作项目(2011DFA30490)

摘　　要：采用ISSR分子标记对舟山群岛、长门岩岛、鹿儿岛、四国岛和五岛的10个山茶(Camellia japonica)居群的遗传多样性和分化程度进行分析。20条随机引物扩增出210个可分析位点,多态性百分比(PPL)为90%。试验结果显示,山茶居群水平的Nei’s基因多样性指数为0.2732,Shannon信息多态性指数为0.4003,表明其具有较高的遗传多样性。鹿儿岛、四国岛和五岛的居群相对于舟山群岛和长门岩岛居群而言,遗传多样性水平更高。10个居群间遗传分化系数Gst=0.2028;地理距离与遗传距离之间具有显著相关性(r=0.9081,P<0.05),表明岛屿地理隔离对山茶居群间的遗传分化具有重要影响。UPGMA聚类显示了居群间的亲缘关系,同岛内居群间亲缘关系更近,长门岩岛居群与舟山群岛居群亲缘关系近于和鹿儿岛、四国岛以及五岛居群间的亲缘关系。Camellia japonica is an important horticultural and economic species, mainly distributed in East Asia. Inter-simple sequence repeat （ISSR） markers were used to investigate the genetic diversity within and among the ten natural populations of Camellia japonica. A total of 210 discernible loci were obtained for all populations using 20 primers, 90% of which were polymorphic （PPL = 90%）. The average value of Nei＇s genetic diversity and Shannon information index of ten populations were 0.2732 and 0.4003, indicated that the genetic diversity of Camellia japonica populations was relatively high. Population in Kagoshima, Shikoku Island and Goto Island had a higher level of genetic diversity than that in Zhoushan Islands and Changmenyan Island. The value of differentiation （Gst = 0.2028） affirmed that Camellia japonica had high genetic differentiation among populations, and a significant correlation was found between genetic distance and geographic distance （r = 0.9081, P 〈 0.05 ） . UPGMA showed that CMY population had a close relationship with Zhoushan Islands populations.

关 键 词：山茶 ISSR 岛屿 遗传多样性 

分 类 号：S685.14[农业科学—观赏园艺]