酿酒酵母细胞表面展示技术在燃料乙醇生产中的应用及研究进展  

Development and application of Saccharomyces cerevisiae cell-surface display for bioethanol production

作　　者：杨非[1] 曹萌[1] 金怡[1] 杨秀山[1] 田沈[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《生物工程学报》2012年第8期901-911,共11页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31100578);国家科技支撑计划课题(No.2011BAD22B01)资助~~

摘　　要：酿酒酵母Saccharomyces cerevisiae细胞表面展示表达系统是一种固定化表达异源蛋白质的真核展示系统,具有糖基化作用及蛋白翻译后折叠等优势,更利于基因工程操作。近年来,酵母细胞表面工程作为一种新兴策略来固定化淀粉水解酶、纤维素水解酶以及木聚糖降解酶,从而应用于燃料乙醇的生产。文中着重介绍了酵母细胞表面展示系统的基本原理、研究现状以及在生物乙醇生产中的应用前景及所面临的挑战。Saccharomyces cerevisiae is useful as a host for genetic engineering,since it allows the folding and glycosylation of expressed heterologous eukaryotic proteins and can be subjected to many genetic manipulations.Recent advancements in the yeast cell surface engineering developed strategies to genetically immobilize amylolytic,cellulolytic and xylanolytic enzymes on yeast cell surface for the production of fuel ethanol from biomass.We reviewed the basic principle and progress of S.cerevisiae cell-surface engineering and gave an insight into the recent technological developments in the production of bioethanol using surface engineered yeast.

关 键 词：酵母细胞表面展示 生物乙醇 淀粉分解酶 纤维素分解酶 统合生物加工 

分 类 号：TQ223.122[化学工程—有机化工]