过表达miR-103促进猪前体脂肪细胞分化  

Over-expressed MiR-103 promotes porcine adipocyte differentiation

作　　者：李美航[1] 仇杨[1] 刘帅[1] 董培越[1] 宁小敏[1] 李艳杰[1] 杨公社[1] 孙世铎[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西杨凌712100

出　　处：《生物工程学报》2012年第8期927-936,共10页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.31072014);西北农林科技大学"创新团队建设计划"资助~~

摘　　要：为阐明miR-103在猪前体脂肪细胞分化过程中的调控作用,采用Real-time PCR检测猪前体脂肪细胞成脂分化过程中的miR-103表达谱,明确了其在分化过程中的表达趋势;使用miR-103的腺病毒超表达载体感染猪原代脂肪细胞,随后采用Real-time PCR和Western blotting分别检测成脂标记基因PPARγ、aP2的mRNA和蛋白表达量变化;油红O染色观察腺病毒miR-103侵染的前体脂肪细胞诱导分化第8天的成脂情况。结果显示,miR-103的表达量随着脂肪细胞分化而增加,在miR-103超表达的猪原代脂肪细胞的诱导分化过程中,成脂标记基因PPARγ、aP2的表达量与对照相比显著升高,分化第8天观察到明显的脂滴。说明miR-103能够促进猪前体脂肪细胞分化。To clarify the function of miR-103 in the differentiation of porcine preadipocyte,we carried out real-time PCR to detect the expression pattern of miR-103 during adipogenesis,and clarified its expression tendency through cell differentiation.Then we used adenovirus that overexpressed miR-103 to infect porcine preadipocyte.Subsequently,mRNA and protein expression of adipogenesis marker—PPARγ and aP2 was analyzed by real-time PCR and Western blotting.At last,Oil-Red O staining was used to detect lipids accumulation in the 8th day after adipogenic inducement.The expression of miR-103 increased during adipocyte differentiation;compared with the control,the preadipocyte infected by pAd-miR-103 had an elevated expression level of adipocyte marker gene PPARγ,aP2,and obvious lipid droplet was seen in the 8th day after adipogenic inducement.These results showed that miR-103 can enhance adipogenesis in primary cultured porcine adipocytes.

关 键 词：miR-103 猪前体脂肪细胞 腺病毒 

分 类 号：S828[农业科学—畜牧学]