PES1与雌激素受体存在相互作用  

PES1 Interacts with Estrogen Receptor

作　　者：李杰萍[1] 庄庆仁 兰小鹏[2] 曾国彬[1] 罗小锋[1] 

机构地区：[1]武警福建总队医院,福州350003 [2]南京军区福州总医院检验医学研究所,福州350003

出　　处：《中国生物工程杂志》2012年第8期14-18,共5页China Biotechnology

基　　金：福建省社会发展科技重点项目(2010Y0048);福建省自然科学基金(2010J05082)资助项目

摘　　要：雌激素(E2)和雌激素受体(ER)在E2诱发的肿瘤中起着极其重要的作用。ER共调节因子通过与ER相互作用调节其生物学功能。PES1主要表达于E2的重要靶器官如乳腺、卵巢等组织中,并在乳腺癌细胞中高表达。用PCR技术构建HA标签的PES1全长以及1～322aa、312～588aa和414～588aa三个不同功能区片段的重组质粒。将不同的重组质粒与FLAG-ERα和或FLAGC-ERβ共转染293T细胞后进行免疫共沉淀,以验证PES1与ER是否有相互作用以及相互作用的区域。用含雌激素受体作用元件的荧光素酶报告基因(ERE-LUC)检测PES1对ERα和ERβ转录激活活性的影响。结果表明,PES1与ERα和ERβ均相互作用,且PES1的1～322aa区域与ERα和ERβ相结合。PES1能特异地、E2非依赖性抑制ERβ的转录激活活性。实验结果显示,PES1是一个新的ER共调节因子,需要进一步研究其在ERβ信号通路及其在E2诱发的肿瘤的作用。Estrogen（E2） and estrogen receptors （ER） play a vital role in the E2-induced neoplasms. The coregulators of ERs modulate their biological functions by binding these receptor. PES1, as a potential regulator, is mainly expressed in the target organs such as breast and ovarian, and also, is highly expressed in the breast cancer ceils. In current work, the HA-tagged recombinant plasmids of full-length PES1, and its different function regions（ 1 -322aa, 312 -588aa and 414 -588aa） were constructed by PCR. To test whether PES1 can interact with ERs and their interaction regions, different recombinant plasmids constructed were co-transfected with FLAG-ERot or/and FLAGC-ERβ in 293T cells before co-immunoPrecipitation （co-IP）. The co-IP results showed that PES1 could interact with ERα and ERβ by binding their 1 - 322aa region. Further experiments demonstrated that PES1 specifically inhibited the transactivation activity of ERβ in E2-independent manner by analyzing estrogen receptor element luciferase （ERE-LUC）. These results suggested that PES1 may act as a new ER coregulator. Further mechanisms and roles in the ERβ signaling pathway and E2-induced neoplasms remain to be determined.

关 键 词：PES1 雌激素受体 共调节因子 

分 类 号：Q789[生物学—分子生物学]