MAR介导的非病毒附着体载体的研究进展  

Research Progress of the Matrix Attachment Mediated Nonvirus Episomal Vector

作　　者：林艳[1] 李照熙[2] 王芳[1] 王天云[1] 

机构地区：[1]新乡医学院生物化学与分子生物学教研室,新乡453003 [2]新乡医学院生命科学技术系,新乡453003

出　　处：《生物技术通报》2012年第8期46-50,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30970055)

摘　　要：附着体载体可以使外源基因在宿主细胞中不整合到基因组上而是以附着体的形式存在,是一种新型的高效、安全、附着体表达载体。目前研究较多的是由S/MAR(Scaffold/Matrix Attachment Region,S/MAR)元件介导的质粒。虽然此类载体均能介导载体附着存在,但是转基因的表达量不同。最近研究发现,载体的启动子、骨架结构、CpG基序以及表达系统等方面能够影响转基因的表达,针对以上内容作一综述,希望据此进一步优化载体,为临床研究提供基础依据。The new type of episomal expression vectors can make transgene expression efficient, continuous, and safety, which do not integrate into the genomes and exist in form of episome in host cells. Currently, more researches focus on episomal vectors which are mediated by scaffold/matrix attachment region components. Though episomal vectors exist in form of episome, there are many differents in the terms of transgene expression. It was found that many factors were affected on transgene expression based on the recent researches, for example, the promoter, the skeleton structure, CpG sequence of the vector, and the express system. Here the development of MAR madiated transgene expression was reviewed. According to it, it is better to optimize the vector furtherly and provide the basis for clinical researches.

关 键 词：核基质附着区 转基因表达 附着体载体 启动子 骨架结构 CPG基序 

分 类 号：R346[医药卫生—基础医学]