转基因水稻中过表达OsHGGT基因提高种子三烯生育酚含量的研究  

Increase of Tocotrienol Content by Overexpression of OsHGGT Gene in Transgenic Rice

作　　者：张桂云[1] 刘如如[1] 张鹏 徐根[1] 朱姜 梁国华[1] 刘巧泉[1] 

机构地区：[1]扬州大学农学院植物功能基因组学教育部重点实验室,扬州225009 [2]国家洗簌用品质量监督检验中心,扬州225009

出　　处：《生物技术通报》2012年第8期64-70,共7页Biotechnology Bulletin

基　　金：国家转基因生物新品种培育重大专项(2011ZX08001-006);江苏省"六大人才高峰"项目

摘　　要：旨在提高稻米中三烯生育酚的含量,将来源于日本晴尿黑酸牻牛儿基牛儿基牻转移酶(homogentisic acid gerany-lgeranyl transferase,HGGT)基因导入粳稻品种武育粳3号过量表达。经PCR和RT-PCR分析证明外源基因已导入水稻中并能够在水稻胚乳中表达。HPLC测定结果表明,过表达HGGT后,转基因水稻种子糠层及胚乳中γ-三烯生育酚和总三烯生育酚的含量分别是未转化对照的1.52和1.67倍,且三烯生育酚的积累并未导致总生育酚含量的降低,最终糠层及胚乳中总三烯生育酚与总生育酚的比值分别提高到0.82和1.82,极显著高于(P<0.01)未转化对照(分别为0.54和1.27)。In present study, with the aim to improve tocotrienol content in rice seed, the eDNA encoding homogentisic acid gerany- lgeranyl transferase （ HGGT ） from Niponbare was over-expressed under the control of CaMV 35S promoter in an elite rice japonica cultivar Wuyujing 3 by Agrobacterium-mediated transformation. The presence and the expression of the input transgene were confirmed by PCR and RT- PCR analyses, respectively, as well as determination of tocopherol and tocotrienol contents by chromatographic analysis. HPLC results showed that tocotriennls in brans and endosperms of transgenie lines increased 1.52 and 1.67 folds compared with the wild type, respectively, and tocotrienols accumulation did not result in decreases of tocopherols content. As a resnh, the ratio of total tocotrienols to total tocopherols in brans or endosperms from transgenic lines were improved up to 0.82 and 1.82, respectively, which were significantly higher （ P〈0.01 ） than those of wild type （ 0.54 and 1.27, respectively ） .

关 键 词：转基因水稻 维生素E 三烯生育酚 尿黑酸牻牛儿基牻牛儿基转移酶牻 

分 类 号：S511[农业科学—作物学]