磁性纳米颗粒作为基因转染载体的研究  

Study on Magnetic Nanoparticles as Carriers for Gene Transfection

作　　者：卢艳敏[1,2] 崔海信[1] 崔金辉[1] 李瑶[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081 [2]衡水学院生命科学系,衡水053000

出　　处：《生物技术通报》2012年第8期199-204,共6页Biotechnology Bulletin

基　　金：转基因生物新品种培育科技重大专项资助项目(2009ZX08010-006)

摘　　要：通过扫描电子显微镜和Zeta电位仪对磁性纳米颗粒的形貌、粒径、表面电位等进行了表征。利用凝胶电泳阻滞试验分析磁性纳米颗粒与DNA的结合情况,研究磁性纳米颗粒对DNA的保护效果,运用MTT和流式细胞术分析磁性纳米颗粒对细胞的毒性。以绿色荧光蛋白基因为报告基因进行293T细胞的转染,研究磁性纳米颗粒与质粒DNA不同比例条件下对293T细胞的转染效率,并与脂质体(Lipofectamine2000)介导的转染进行比较分析。结果表明,磁性纳米颗粒与DNA可以稳定结合,可以保护DNA免受酶的消化作用,当磁性纳米颗粒与DNA比为1 1时,转染效率最高,优于脂质体(Lipotamine2000)介导的转染,且对细胞的毒害作用小于Lipotamine2000。The appearance, particle size, surface potential of magnetic nanoparticles （ MNPs ） were characterized by scanning electron microscopy and zeta potential analyzer. Combination ability, protection ability of MNPs to DNA, toxicity to cells was investigated by gel retardation assay, protection assay of magnetic nanoparticles against DNase I, MTT and flow cytometer assay. Transfection efficiency to 293T cells mediated by MNPs with GFP gene as reporter gene were examined, Lipofectamine20OO-mediated transfection were used as control. The results show that magnetic nanoparticles can combine with DNA stably, can protect DNA from enzymatic digestion. Transfection efficiency mediated by MNPs were better than Lipotamine2000 mediated transfection and toxic of MNPs/DNA complex to cells was less than Lipotamine2000/DNA complex when the ratio of magnetic nanoparticles to DNA is 1 ： 1.

关 键 词：磁性纳米颗粒 基因载体 转染 

分 类 号：Q78[生物学—分子生物学]