丹芍化纤胶囊对肺纤维化大鼠TGF-β_1及Smad_(3/7)表达的影响  

Effect of Danshao Huaxian capsule on the expression of TGF-β_1 and Smad_(3/7) in the lung in rats with fibrosis pulmonary

作　　者：何小飞[1,2] 吴艳琦[1] 杨勤[2] 

机构地区：[1]贵阳医学院病理生理教研室,贵州贵阳5500042 [2]黔南民族医学高等专科学校,贵州都匀558003

出　　处：《华西药学杂志》2012年第4期410-412,共3页West China Journal of Pharmaceutical Sciences

基　　金：社发攻关项目[黔科合SZ字(2008)3049]

摘　　要：目的观察丹芍化纤对博来霉素致肺纤维化大鼠肺组织中TGF-β1及Smad3/7表达的影响。方法将30只SD大鼠,随机均分为对照组、模型组和治疗组;给予模型组和治疗组大鼠的气管内注射博来霉素(5 mg·kg-1)诱导肺纤维化,对照组的气管内注射等量生理盐水;次日起ig给予治疗组大鼠丹芍化纤混悬液(0.8 g·kg-1·d-1),其余两组ig给予等量生理盐水;28 d后处死,观察各组大鼠肺组织病理变化;运用免疫组化法观察肺组织中TGF-β1及Smad3/7表达的变化。结果模型组大鼠的肺系数明显增加,肺组织中胶原沉积明显,羟脯氨酸含量、TGF-β1和Smad3的表达增加,而Smad7表达下降;与模型组比较,治疗组大鼠的肺系数明显降低,肺组织中胶原沉积有所减轻,羟脯氨酸的含量、TGF-β1和Smad3蛋白的表达减少,而Smad7表达升高。结论丹芍化纤具有较好的抗大鼠肺纤维化作用,其机制可能与丹芍化纤影响TGF-β1/Smads信号转导通路、下调TGF-β1及Smad3的表达,同时上调Smad7的表达有关。OBJECTIVE To investigate the effect of Danshao Huaxian capsule（DSH） on TGF-β1 and Smad3/7 expression in rats with pulmonary fibrosis.METHODS Thirty Spague-Dawley rats were randomly divided into the control group,model group and test group.The model group and the test group were induced pulmonary fibrosis with endotracheal injection of bleomycin（5 mg·kg-1）,while the control group was given normal saline instead.From the second day of injection,the rats in the test group were administered intragastricly with DSH（0.8 g·kg-1·d-1）,while the other rats were given normal saline instead.All rats were sacrificed at the twenty-eighth day,and the pathological changes in rats were detected.The expression of TGF-β1 and Smad3/7 were examined by immunohistochemical staining.RESULTS In model group,the lung coefficient,the Hyp content,expressions of TGF-β1 and Smad3 were significantly increased,while the expression of Smad7 was decreased.Compared with model group,the lung coefficient were significantly decreased,pulmonary alveoli inflammation and pulmonary fibrosis were relieved,the Hyp content and expression of TGF-β1 and Smad3 were obviously decreased in the test group,but Smad7 was significantly increased.CONCLUSION DSH has a good effect against experimental pulmonary fibrosis in rats.The mechanism may be associated with the influence of DSH on TGF-β1/Smads signal transduction with down-regulating the expressions of TGF-β1 and Smad3 while up-regulating the expression of Smad7.

关 键 词：肺纤维化 丹芍化纤 TGF-β1 SMAD3 SMAD7 

分 类 号：R96[医药卫生—药理学]