苯致模拟失重大鼠肝细胞DNA-蛋白质交联的研究  

Study on DNA-protein Crosslinks Induced by Benzene in the Liver of Rats Weightlessness Simulated by Tail-suspension

作　　者：李曙光[1] 何新星[1] 吴大蔚[1] 杨成佳[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《航空航天医学杂志》2012年第8期932-934,共3页Journal of Aerospace medicine

基　　金：载人航天领域预先研究项目(编号:010204)

摘　　要：目的:探讨苯吸入染毒对尾吊模拟失重大鼠肝细胞DNA-蛋白质交联(DNA-protein crosslinks,DPC)作用。方法:将30只雄性SD大鼠随机分为3组:对照组和2个染毒组。对照组吸入新鲜空气,染毒组分别给予浓度为10 mg/m3和50 mg/m3的苯气体进行吸入染毒,每天24 h,连续7 d。结果:与对照组相比,低浓度的气态苯(10 mg/m3)可以诱导显著的DNA-蛋白质交联作用(P<0.05),高浓度的气态苯(50 mg/m3)可以诱导极显著的DNA-蛋白质交联作用(P<0.01)。结论:高低浓度苯吸入染毒均可致肝细胞内的DPC系数显著上升,且随苯浓度上升,细胞中的DPC含量出现极显著上升。Objective： To explore effects of benzene exposure on DNA - protein crosslinks in the Liver of rats weightlessness simulated by tail - suspension. Methods： Thirty Male SD rats ware divided randomly into three groups, control group with air and benzene group with breathed at the dose of 10 mg/m3 and 50 mg/m3 benzene for 7 days. Results：As compared with control group, gaseous benzene at low concentrations （10 mg/m3） results in insignificantly increase in DPC. High concentrations （10 mg/m3） lead to especial insignificantly increase in DPC. Conclusions： The experiment results showed that benzene exposure could induce DPC at high and low concentration, with benzene concentration incease, the amount of DPC relatively ascend.

关 键 词：苯 尾吊KCl-SDS沉淀法 DNA-蛋白质交联 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]