溴氰菊酯对德国小蠊滞留喷洒药效与kdr突变关系研究  

Residual spray effect of deltamethrin on Blattella germanica and its relationship with kdr mutation

作　　者：周小洁[1] 刘婷[1] 钱坤[1] 佟颖[1] 曾晓芃[1] 

机构地区：[1]北京市疾病预防控制中心消毒与有害生物防制所,北京100013

出　　处：《中国媒介生物学及控制杂志》2012年第4期310-313,共4页Chinese Journal of Vector Biology and Control

摘　　要：目的明确现场种群德国小蠊对溴氰菊酯抗性状态,研究其滞留喷洒的杀蟑效果,以及耐受个体kdr抗性基因型。方法根据相关国标用药膜法检测试虫对溴氰菊酯抗性,用强迫接触法测定在玻璃、清漆木板及水泥3种代表性表面滞留喷洒药效,用钠离子通道基因DNA扩增产物测序确定基因型。结果随着表面吸水性增强,对试虫击倒速度降低,持效期缩短;不同种群的抗性状态影响药效,3种表面对敏感及低抗(2.05倍)种群均有较好效果,对中抗(5.17倍)种群效果较差。对杀虫剂耐受的个体均携带kdr抗性基因型,中抗种群中抗性纯合(RR)基因型频率较高,而低抗种群中抗性杂合(RS)基因型频率较高。结论滞留喷洒防治中,德国小蠊对杀虫剂抗性表型与其携带的抗性基因型密切相关,不恰当的防治反而增加种群中抗性基因频率,因此要根据环境类型、靶标抗性状态与用药史,调整施药次数与剂量,并将滞留喷洒与胶、毒饵等手段结合,从而达到理想的防治效果。Objective To determine the residual spray effect of deltamethrin on German cockroach （Blattella germanica） and the potential relationship between the kdr mutation and the resistance. Methods The resistance ratio of the two field strain B. germanica to deltamethrin was detected by residual contact （glass jar）. The forced contact test was conducted to detect the residual spray effect on 3 representative panels of glass, painted wood and cement. The kdr genotype was determined by sequencing. Results The knockdown rate decreased with the increase in the surface water absorption, with the duration of efficacy shortened. The killing effect of the insecticide varied with the resistance status of different strains. The 3 panels were all able to kill the susceptible and low resistance strains effectively, but had no reliable effect on the strain with moderate resistance. Low and moderate resistant field strains that were tolerant to deltamethrin all had the kdr genotype, with the former having a relatively high frequency of heterozygosis genotype （RS）, the latter relatively high in the homozygosis genotype （RR）. Conclusion B. germanica resistance to deltamethrin residual spray was closely related to the kdr genotypes. Incorrect use of insecticide may result in the increase of kdr genotype frequency, so frequency and dosage need to be adjusted based on the environment types, the target resistance and the insecticide history with residual spray management combined with toxic or gel bait to achieve the ideal control effect.

关 键 词：滞留喷洒 溴氰菊酯 德国小蠊 击倒抗性 基因型 

分 类 号：R384.9[医药卫生—医学寄生虫学] S481.9[医药卫生—基础医学]