小鼠外周血心肌特异性microRNAs的变化与病毒性心肌炎相关关系初探  

Identification of Mice Specific MicroRNA in Peripheral Blood during the Process of Vital Myocarditis

作　　者：黄丽颖[1] 肖宗慧[1] 姚海兰[1] 何峰[1] 张庆华[1] 刘哲伟[1] 

机构地区：[1]首都儿科研究所分子免疫室,北京100020

出　　处：《现代生物医学进展》2012年第20期3831-3834,共4页Progress in Modern Biomedicine

基　　金：北京市自然科学基金(7102023);北京市优秀人才资助项目(2011A003034000032)

摘　　要：目的:通过识别病毒性心肌炎小鼠和正常小鼠血浆中miR-1,miR-133,miR-206表达量的差异,分析外周血中心肌特异性microRNAs的变化与病毒性心肌炎相关关系,为探索miRNAs作为病毒性心肌炎诊断的生物标志物提供可行性的研究资料。方法:在小鼠病毒性心肌炎模型的基础上,采用荧光定量PCR方法,检测病毒性心肌炎急性期小鼠组和正常小鼠组血浆中相关miRNAs含量,并进行统计学分析。再于病毒注射后1d,3d,5d,7d,9d,11d,分别处死病毒小鼠,观察血浆miRNAs的动态变化规律。同时用Elisa检测心肌肌钙蛋白的变化,对目的 miRNAs与心肌肌钙蛋白进行相关性分析。结果:急性期时三种miRNAs血浆含量显著上调。病毒注射后3d开始上升明显,并持续保持在较高水平到7d,于9d时开始下降。发病期间,血浆miRNAs含量与心肌肌钙蛋白呈现良好的正相关性。结论:小鼠外周血中心肌特异性microRNAs在病毒性心肌炎发病过程中的呈现明显上调并与病程和相应指标存在相关关系,为miRNAs作为病毒性心肌炎诊断的生物标志物提供了重要线索。Objective：To investigate the possibility of circulating microRNAs as the biomarkers for diagnosis of acute viral myocarditis by detecting the variability expression of miR-1,miR-133,miR-206 between acute viral myocarditis mice and healthy mice.Methods： Mice viral myocarditis model was established.The variability expression of miR-1,miR-133,miR-206 between acute viral myocarditis mice and healthy mice was detected by real-time PCR,then process statistical analysis.Mice were killed at 1d,3d,5d,7d,9d,11d,and the plasma miRNAs dynamic change regulation was observed.The change of cardiac troponin was detected simultaneously,and the correlation between miRNAs and cardiac troponin was detected.Results： Three miRNAs plasma rose significantly in acute phase.3 days after virus injection,microRNAs started to rise significantly,and maintained at high levels until the 7th day,on the 9th day began to fall.The level in plasma of the 3 miRNAs was positively correlated with cardiac troponin.Conclusions： It is possible for specific miRNAs miR-1,miR-133,miR-206 in myocardial cells to be considered a new class of biomarkers for diagnosis of clinical disease.

关 键 词：病毒性心肌炎 血浆 MIRNAS 实时定量PCR 

分 类 号：R542.21[医药卫生—心血管疾病]