仙灵骨葆对骨质疏松大鼠OPG/RANK/RANKL表达的影响  

Effect of Xianlinggubao on the Expression of OPG/RANK/RANKL in Osteoporotic Rats

作　　者：冯建书[1] 付强[1] 闫世杰[1] 张乐明[1] 王江泳[1] 周晓彬[1] 王海涛[1] 

机构地区：[1]石家庄市第三医院骨科,河北石家庄050011

出　　处：《现代生物医学进展》2012年第23期4438-4441,4437,共5页Progress in Modern Biomedicine

基　　金：石家庄市科技局(0512038)

摘　　要：目的:观察仙灵骨葆治疗骨质疏松模型大鼠后,对大鼠体内OPG/RANKL/RANK表达的影响。方法:卵巢摘除法建立SD大鼠骨质疏松模型,设立假手术组、对照组(单纯去卵巢组)、雌激素组(给予17β-雌二醇)和治疗组(给予仙灵骨葆)。术后1周开始给药,给药12周后检测各组大鼠股骨骨密度,ELISA法检测血清中OPG/RANKL含量,RT-PCR检测骨组织中OPG/RANK/RAN-KL mRNA表达,免疫组化检测骨组织中RANK的表达。结果:对照组大鼠骨密度显著低于假手术组;治疗组和雌激素组大鼠O-PG表达显著高于对照组,RANK及RANKL的表达显著低于对照组。结论:采用卵巢摘除法成功建立大鼠骨质疏松模型;仙灵骨葆可促进骨质疏松大鼠OPG的表达,并抑制RANK及RANKL的表达,对骨质疏松模型大鼠有治疗作用。Objective： To investigate the impact of Xianlinggubao treatment on OPG/RANKL/RANK expression in osteoporosis rats. Methods： SD rat model of osteoporosis was established by ovariectomy and divided into four groups： the sham operation group, the control group （OVX simply）, the estrogen group （given 17B-estradiol） and treatment group （given Xianlinggubao）. The rats were administered one week after surgery. After 12 weeks dosing, all rats had their bone mineral density detected. The OPG/RANKL content in serum were detected by ELISA, and the OPG/RANKL/RANK mRNA expression in bone tissue were detected by RT-PCR, and the RANK expression in bone tissue were detected by immunohistochemical assay. Results： The bone mineral density in control group was significantly lower than the sham operation group; The OPG expression in treatment/estrogen group were significantly higher than the control group; The RANK and RANKL expression in treatment/estrogen group were significantly lower than the control group. Conclusion： A rat model of osteoporosis was successfully established by the ovariectomy method; Xianlinggubao can promote the expression of OPG and inhibit the expression of RANK/RANKL in osteoporosis rats. It has a therapeutic role in the rat model of osteoporosis.

关 键 词：骨质疏松 仙灵骨葆 OPG RANK RANKL 

分 类 号：Q95-3[生物学—动物学] R68[医药卫生—骨科学]