不同剂量乙醇对地西泮及氯胺酮用药小鼠学习记忆的影响  

The effects of different doses of ethanol on learning and memory in mice with diazepam and ketamine administration

作　　者：陈慧娟[1] 虞黎黎[1] 曹兆成[1] 戴平[1] 庞步军[1] 张妤[1] 张咏梅[2] 

机构地区：[1]徐州医学院麻醉学院2009级,江苏徐州221004 [2]徐州医学院麻醉生理学教研室,江苏徐州221004

出　　处：《徐州医学院学报》2012年第7期424-427,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（81171041）;江苏省自然科学基金（BK2011197）

摘　　要：目的研究不同剂量乙醇对地西泮和氯胺酮用药小鼠学习记忆的影响。方法按分层随机区组设计将80只小鼠分为10组（每组n=8）：生理盐水＋地西泮（A组）；10％乙醇＋地西泮（B组）；20％乙醇＋地西泮（c组）；40％乙醇＋地西泮（D组）；生理盐水＋氯胺酮（E组）；10％乙醇＋氯胺酮（F组）；20％乙醇＋氯胺酮（G组）；40％乙醇＋氯胺酮（H组）；生理盐水＋地西泮＋氯胺酮（I组）；10％乙醇＋地西泮＋氯胺酮（J组）。用避暗实验测定各组用药后1、24、48h的潜伏期和错误次数。结果与A组相比，D组错误次数减少（P〈0．05），但D组小鼠处于昏睡状态，表明乙醇对中枢高度抑制。与B组相比，c组潜伏期缩短、错误次数增多（P〈0．05），表明随着乙醇浓度的增高，乙醇对地西泮用药小鼠学习记忆的抑制作用增强。与E组相比，F组和G组潜伏期缩短（P〈0．05），表明乙醇可以增强氯胺酮对小鼠学习记忆的抑制作用。与c组相比，G组错误次数减少、潜伏期延长（P〈0．05），表明乙醇对地西泮用药小鼠学习记忆的抑制作用强于氯胺酮。结论20％浓度的乙醇对地西泮用药小鼠学习记忆的抑制作用强于氯胺酮。Objective To observe the effects of different doses of ethanol on learning and memory in mice with dia- zepam and ketamine administation. Methods In a stratified and random block design, 80 mice were divided into 10 groups （n =8 each） ： saline ＋ diazepam （group A） ; 10% ethanol ＋ diazepam （group B） ; 20% ethanol ＋ diazepam （group C） ; 40% ethanol ＋ diazepam （group D） ; saline ＋ ketamine （group E） ; 10% ethanol ＋ ketamine （group F）; 20% ethanol ＋ ketamine （group G）; 40% ethanol ＋ ketamine （group H）; saline ＋ diazepam ＋ ketamine （group I） ; 10% ethanol ＋ diazepam ＋ ketamine （group J）. The step- through test was performed to observe latency and error times in each group at 1, 24 and 48 h after drug administration. Results Compared with group A, the error times in group D decreased , but the mice of group D was in a state of lethargy, indicating that ethanol inhibited the central nervous system highly. Compared with group B, the latency in group C decreased and error times increased （P 〈 0.05 ） , indicating that with the increase of the concentration of ethanol, the inhibition effect of ethanol on learning and memory in mice with diazepam administration enhanced. Compared with group E, the latency in group F and group G de- creased （ P 〈 0.05 ）, indicating that ethanol could enhance the inhibition effect of learning and memory in mice with ket- amine administration. Compared with group C, the error times in group G decreased and the latency increased （P 〈 0.05 ） , indicating that the inhibition effect of ethanol on learning and memory in mice with diazepam administration was stronger than ketamine. Conclusion The inhibition effect of 20% ethanol on learning and memory in mice with diaze- pare administration is stronger than ketamine.

关 键 词：乙醇 地西泮 氯胺酮 学习记忆 避暗实验 小鼠 

分 类 号：R614[医药卫生—麻醉学] R971[医药卫生—外科学]