小佛肚竹生氰糖苷合成关键酶CYP79家族同源基因的克隆和鉴定  

Cloning and characterization of the CYP79 homolog-key enzyme gene involved in biosynthesis of cyanogenic glycosides in Bambusa ventricosa

作　　者：王月圆[1] 刘向敏[1] 周明兵[1] 汤定钦[1] 

机构地区：[1]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《浙江农林大学学报》2012年第4期510-515,共6页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(31070590;R303420)

摘　　要：生氰糖苷是由氰醇衍生物的羟基和D-葡萄糖缩合形成的糖苷,已在2 000多种植物中发现,分属于蕨类植物、裸子植物和被子植物的130个家族。生氰糖苷的主要生物学功能在于阻止食草动物和病原体对植物的侵害。通过反转录-聚合酶链式反应(RT-PCR)和cDNA末端快速扩增(RACE)技术从小佛肚竹Bambusa ventricosa幼笋中克隆到CYP79家族同源基因的cDNA全长,命名为BvCYP79。BvCYP79共有1 913个碱基对,翻译起始点为193碱基处,终止子位于1 752 bp,包含1个1 559 bp的完整编码区,开放阅读框共编码519个氨基酸。具有PERF和SFSTGRRGCIA保守结构域,属于典型的单子叶植物CYP79家族基因。生氰糖苷途径可能也存在于竹类植物,因而在食用笋的安全性评估上具有一定指导意义。Cyanogenic glycosides, a molecule in which a sugar is bound to a non-carbohydrate moiety, usually a small organic molecule, and found in more than 2 000 higher plant species, including ferns, gymnosperms, and angiosperms making up 130 plant families, have been used as defense compounds against herbivores and pathogens. In order to investigate whether there is cyanogenic glycosides pathway in the Bambusa ventricosa, the CYP79 homolog gene was aimed to characterized. In this study, a CYP79 homolog gene （named BvCYP79） was cloned from the shoot tip of B. ventricosa McClure by reverse transcription-polymerase chain reaction （RT-PCR） and rapid amplification of cDNA ends （RACE）, which was 1 913 base pairs （bp） in length encoding 519 amino acids. The start codon was located at 193 bp, and the stop codon was located at 1 752 bp. Results showed that the translated BvCYP79 contained the typical plant CYP79 domains including PERF and SFSTGRRGCIA motifs. The obtained data showed a possible cyanogenic glucoside pathway in B. ventricosa by blast and functional prediction. This could be helpful for safety evaluation with bamboo shoots. [Ch, 3 fig. 12 ref.]

关 键 词：分子生物学 生氰糖苷 小佛肚竹 CYP79家族 

分 类 号：S718.3[农业科学—林学] Q943.2[生物学—植物学]