补髓生血颗粒对慢性再障患者骨髓单个核细胞内Ca^(2+)水平及不同血液疾病间差别的影响  

Impact of Ca^(2+) Concentration in Bone Marrow Mononuclear Cells with Busuishengxue Granule in Chronic Aplastic Aaenia Patients and the Observation of It in Different Blood Diseases

作　　者：罗正凯[1] 王金环[2] 孙凤[2] 雍延礼[2] 孙伟正[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《中医药信息》2012年第4期53-55,共3页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(90709039);教育部博士学科点基金项目(200802280003)(20092327120001);中国博士后基金项目(20100481034);黑龙江省中医药管理局基金项目(ZHYO-W42);黑龙江中医药大学基金项目(200901)

摘　　要：目的:通过观察慢性再障患者骨髓单个核细胞内钙离子浓度的变化,探讨补髓生血颗粒对慢性再生障碍性贫血(CAA)的可能治疗机制。观察不同血液疾病骨BMMC内Ca2+浓度的变化,以期为血液疾病的诊断和治疗提供思路。方法:提取CAA患者BMMC,使用fluo3 AM进行荧光染色,采用流式细胞仪检测细胞内Ca2+浓度。同时提取慢性再障、骨髓增生异常综合征、特发性血小板减少性紫癜、缺铁性贫血患者及正常志愿者BMMC,进行细胞内Ca2+浓度的检测与对比。结果:CAA患者BMMC内Ca2+浓度明显高于正常组。经补髓生血颗粒治疗后,各组患者细胞内Ca2+浓度均出现下降,治疗组优于对照组,但仍高于正常组。MDS、CAA患者Ca2+浓度高于其他组;ITP、IDA组与正常组无明显差异。结论:CAA患者BMMC细胞内Ca2+浓度上升,是CAA患者疾病情况的重要标志。补髓生血颗粒能调节BMMC内Ca2+的释放,可能是其治疗CAA的作用机制之一。MDS、CAA、CITP、IDA不同血液疾病之间钙离子浓度存在明显差异,可能与疾病恶性程度及发病机理相关。Objective：To observe the change of Ca2＋ concentration in bone marrow mononuclear cells（BMMC） in chronic aplastic anemia（CAA） , exploring the possible mechanism of Busui Shengxue particles on CAA. To explore diagnosis and treatment ideas by observing the Ca2＋ concentration in different blood diseases. Method： Extraction separation the CAA patients＇ BMMC. Use fluo3 AM staining by flow cytometry detection of intracellular calcium concentration. Detect the Ca2＋ concentration in CAA, Myelodysplastic Syndrome（MDS） , Idiopathic Thrombocytopenic Purpnra（ITP）, Iron -Deficiency Anemia（IDA） and compare them. Results： CAA patients＇ BMMC intracellular Ca2＋ concentrations were significantly higher than those in the normal group. After Busui Shengxue particles treatment, patients in each group of intracellular Ca2＋ concentration declined, Busui Shengxue particle group was better than the control group, but still higher than the normal group. MDS and CAA was higher than others. ITP, IDA and normal group were no significant difference. Conclusion： The concentration of BMMC intracellular Ca2＋ rise is an important symbol of CAA disease situation. The Busui Shengxue particles can regulate Ca2＋ release in BMMC, which may be one mechanism of the treatment of CAA. MDS,CAA,CITP, IDA＇ s Ca2＋ concentrations have significant differences, which may be related to the disease＇ s degree of malignancy and mechanism.

关 键 词：贫血 再生障碍性 慢性 CA2+ 

分 类 号：R259[医药卫生—中西医结合]