抗痫灵对癫痫大鼠脑电图及海马NMDAR1表达的影响  

Effect of Kangxianling on the EEG in Brains and the Expression of NMDAR1 in Hippocampus of Epilepsy Rats

作　　者：孙丽[1,2] 冯铁为[2] 李敬孝[2] 

机构地区：[1]大庆龙南医院,黑龙江大庆163453 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2012年第4期89-90,共2页Information on Traditional Chinese Medicine

摘　　要：目的:观察中药复方抗痫灵对戊四氮点燃癫痫大鼠模型脑电图及海马NMDAR1表达的影响。方法:Wistar雄性大鼠60只,体质量180～220g,随机分成空白组、模型组、抗痫灵高、中、低剂量组、丙戊酸钠(VPA)组各10只。点燃后各组大鼠描记脑电图,低温条件下迅速断头取脑,用免疫印迹(Westernblot)方法观察各实验组大鼠海马的NMDAR1活性变化。结果:抗痫灵组、丙戊酸钠组、模型组阳性细胞表达与空白组相比有显著性差异(P<0.05),抗痫灵组显著低于模型组(P<0.01)。结论:抗痫灵对戊四氮点燃大鼠癫痫发作有显著对抗作用,其作用机制可能与其降低模型大鼠海马NMDAR1活性有关。Objective ： To observe the effect of Kangxianling on the EEG and the expression of NMDAR1 in hip- pocampus of the PTZ - induced epilepsy model of rats. Methods ： PTZ was used to evoke chronic epilepsy model. The Wister rats were randomly divided into control group, PTZ group ; low - dose Kangxiangling group; moderate- dose Kangxianling group;high -dose Kangxianling group;VPA group. Ethology changes and electro encephalography were observed after epileptic seizure, expressions of NMDAR1 were investigated with Western blot method. Results：The group of Kang xianling, VPA group and PTZ group were significantly different from the control group （ P 〈 0. 05 ）. The group of Kangxianling was siginificantly different from the model group （ P 〈 0. 01 ）. Conclusion ： Kangxianling has an anti - epileptic seizure resistant function on PTZ - induced epilepsy-model of rats. Its functional mechanism may be to react by reducing the content of NMDAR1 in the hippocampus.

关 键 词：抗痫灵 癫痫 戊四氮 NMDAR1 

分 类 号：R285.5[医药卫生—中药学]