桦菌芝多糖对S180瘤组织p53蛋白表达的影响  

Effect of Pyropolyporus Fomentaries Polysaccharide on p53 Protein Expression of Sarcoma S180 Tissue

作　　者：郭丽新[1,2] 王世龙[2] 冯涛[2] 

机构地区：[1]东北林业大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药信息》2012年第4期152-153,共2页Information on Traditional Chinese Medicine

基　　金：黑龙江省教育厅项目(11521266)

摘　　要：目的:观察桦菌芝多糖对小鼠移植型肉瘤(S180)的抑制作用及对瘤组织p53蛋白表达的影响。方法:建立S180小鼠动物模型,取瘤组织称其质量,计算抑瘤率;用链霉菌抗生素蛋白-过氧化酶免疫组化法来测定瘤组织中p53蛋白含量,通过多媒体图像分析系统分析实验结果。结果:桦菌芝多糖高、中剂量组抑瘤率分别为41.84%、41.13%,桦菌芝多糖高、中剂量组S180瘤组织突变型p53蛋白表达减少,与模型组比较差异显著(P<0.05)。结论:桦菌芝多糖对小鼠肉瘤S180有抑制作用,可下调小鼠S180瘤组织突变型p53基因表达。Objective： To study the effect on p53 protein expression in S180 tumor tissue with Pyropolyporus Fomentaries polysaccharide （PFPS） and inhibition of sarcoma - S180 cell proliferation of mice. Methods： The tumor weight was measured to calculate the tumor inhibition rate. Immunohistochemistry method was used to detect p53 protein in tumor tissue treated with PFPS. Results： The PFPS treatment at 100 and 50mg/kg re- sulted in tumor growth inhibition rates of 41.84% and 41.13%. Immunohistochemistry analysis demonstrated the expression level of mutatant p53 decreased. There was a significant difference compared with the negative control group（P 〈0. 05）. Conclusion：The PFPS has an inhibitory effect against S180 sarcoma in vivo and can decrease the expression level of mutatant p53.

关 键 词：桦菌芝多糖(PFPS) 小鼠移植型肉瘤 P53 

分 类 号：R285.5[医药卫生—中药学]