结核菌噬菌体生物扩增法与结核菌DNA联合检测对痰菌阴性肺结核诊断的价值  

THE DIAGNOSTIC VALUE OF ASSOCIATED DETECTION OF PHAGE BIOAMPLIFICATION AND TB-DNA IN PULMONARY TUBERCULOSIS WITH NEGATIVE SPUTUM CULTIVATIONS

作　　者：李振生[1] 李德新[2] 陈素丽[1] 李卫红[1] 李幸彬[1] 刘锐[1] 

机构地区：[1]河北省胸科医院结核科,河北石家庄050041 [2]河北省胸科医院ICU,河北石家庄050041

出　　处：《河北医科大学学报》2012年第8期881-883,共3页Journal of Hebei Medical University

基　　金：河北省科技厅科技支撑计划项目(09276176)

摘　　要：目的探讨痰结核分枝杆菌噬菌体生物扩增法(tuberculosis bacterium phage bioamplification,TB-PhaB)与痰结核菌脱氧核糖核酸(tuberculosis bacterium dcoxyribosc nucleic acid,TB-DNA)联合检测对痰菌阴性肺结核诊断的价值。方法选取初治痰菌阴性肺结核患者132例,进行TB-PhaB与TB-DNA检测。结果对于痰菌阴性肺结核,TB-PhaB检测的敏感度是51.52%、特异度是97.37%;TB-DNA检测的敏感度是62.88%、特异度是90.79%;TB-PhaB与TB-DNA并联联合检测的敏感度是82.58%、特异度是89.40%。结论对于痰菌阴性肺结核,TB-DNA或TB-PhaB单独应用容易漏诊;TB-PhaB与TB-DNA联合检测可为痰菌阴性肺结核的早期诊断提供重要的临床参考价值。Objective To investigate the diagnostic value of associated detection of phage bioamplification （phaB） and tuberculosis bacterium deoxyribose nucleic acid （ TB - DNA） in pulmonary tuberculosis with negative sputum smear and culture. Methods A total of 132 cases of initial treated pulmonary tuberculosis with negative sputum were detected with PhaB and TB - DNA. Results For the sputum negative pulmonary tuberculosis, the sensitivity of TB - PhaB detection was 51.52%, specificity was 97.37% ;the sensitivity of TB - DNA detection was 62.88%, specificity was 90.79% ;the sensitivity of TB - PhaB and TB - DNA combined detection was 82.58%, specificity was 89.40%. Conclusion For the sputum negative pulmonary tuberculosis, single application of TB - PhaB or TB - DNA can easily result in misdiagnosis. TB - PhaB and TB - DNA combined detection proves valuable in providing important clinical reference for the early diagnosis of sputum negative pulmonary tuberculosis.

关 键 词：细菌噬菌体 结核 诊断 

分 类 号：R521[医药卫生—内科学]