利用酵母双杂交系统鉴定玉米Pto蛋白与Pti1蛋白间的相互作用  

Interactions Between Pto and Pti1 Proteins from Maize by the Yeast Two-Hybrid System

作　　者：邹华文[1] 宋仲戬[1] 朱泳[1] 

机构地区：[1]长江大学农学院,湖北荆州434025

出　　处：《玉米科学》2012年第4期42-44,49,共4页Journal of Maize Sciences

基　　金：国家自然科学基金(30700433);湖北省杰出青年基金(2010CDA096);公益性行业(农业)科研专项(201203032)

摘　　要：玉米类Pto以及类Pti1基因参与多种逆境胁迫的信号传导。本研究分别将玉米ZmPto基因、ZmPti1a和ZmPti1b基因插入酵母表达载体pEG202和pJG4-5中,利用酵母双杂交的方法验证玉米类Pto以及类Pti1蛋白的相互作用。结果表明,无论是携带空载体还是携带一个(ZmPti1或ZmPto)融合蛋白的菌株都不能激活报告基因,只有同时携带ZmPti1和ZmPto蛋白的菌株可以激活报告基因,初步确定ZmPto蛋白与ZmPti1蛋白存在相互作用。Pto-like and Pti1-like genes from maize play important roles in stress signal transduction.In this study,the ZmPto and ZmPti1a,ZmPti1b were inserted into yeast expression vectors pEG202 and pJG4-5,respectively,to check their interactions via yeast two-hybrid system.The results showed that yeast strains neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPti1 or ZmPto can activate reporter genes.Only the yeast strains carrying both fusion sequence of ZmPti1 and ZmPto can activate reporter genes indicating that ZmPto can interact directly with ZmPti1 in the yeast two-hybrid system.

关 键 词：玉米 酵母双杂交 生物学鉴定 

分 类 号：S513[农业科学—作物学]