香青兰及其含药血清对H9c2心肌细胞三种缺氧/复氧损伤模型的影响  

Effect of ethanol extracts from Dracocephalum moldavica L. and its medicinal serum on three types of hypoxia/reoxygenation injury in H9c2 cardiomyocytes

作　　者：田友清[1,2] 尚靖[1] 阿布卡德[1] 

机构地区：[1]中国药科大学新药筛选中心,南京210009 [2]江苏联合职业技术学院连云港中医药分院,连云港222007

出　　处：《中国新药杂志》2012年第15期1736-1739,1748,共5页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2011ZX09401-007);国家科技支撑计划(2012BAI30B00);中央高校基本科研业务费专项基金(JKY2009025)

摘　　要：目的:比较香青兰醇提物及其含药血清对H9c2心肌细胞3种缺氧/复氧损伤模型的影响,考察香青兰对心肌缺血/再灌注损伤的保护作用。方法:体外培养H9c2心肌细胞,以Na2S2O4,N2和厌氧袋为缺氧环境分别建立缺氧/复氧损伤模型,以T-SOD,MDA,LDH为指标,考察香青兰醇提物(1,10,100μg.mL-1)及其含药血清对H9c2心肌细胞缺氧/复氧损伤的影响。结果:与模型组比较,香青兰醇提物及其含药血清能明显抑制缺氧/复氧损伤H9c2心肌细胞LDH释放和MDA含量,升高T-SOD活力(P<0.05或0.01)。结论:香青兰醇提物及其含药血清对H9c2心肌细胞缺氧/复氧损伤具有保护作用,提示香青兰具有保护心肌缺血/再灌注损伤的作用。Objective： To investigate the effect of ethanol extracts from Dracocephalum moldavica L （DME） and its medicinal serum on three types of hypoxia/reoxygenation （H/R） injury in H9c2 cardiomyocytes, and to ensure its protective action against myocardial ischemia/reperfusion injury （MIRI）. Methods： Three differ- ent H/R models were established with Na2S204, N2 or anaeroPouch-Anaero in H9c2 cells. T-SOD activity, MDA level and leakage of LDH were assessed to evaluate the effect of DME and its medicinal serum on H/R injury in H9c2 cells. Results： Compared with control, DME （ 1, 10 and 100 μg.mL^（-1） ） and its medicinal serum after orally administration increased T-SOD activity in H9c2 cells during H/R injury, and inhibited LDH leakage and MDA level （P 〈 0.05 or 0.01 ）. Conclusion ： DME and its medicinal serum have a protective effect against H/R injury in H9c2 cells, suggesting that Dracocephalum moldavica L. may have protective effect on MIRI.

关 键 词：香青兰 血清药理学 H9C2心肌细胞 缺氧/复氧损伤 心肌缺血/再灌注损伤 

分 类 号：R961[医药卫生—药理学]