GC内标法同时测定刺山柑果实中3种刺激性成分的含量  

Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method

作　　者：郭小娜[1] 陈文[1] 张华[1] 任全霞[1] 刘珊珊[1] 

机构地区：[1]新疆石河子大学药学院,新疆石河子832000

出　　处：《中国实验方剂学杂志》2012年第16期66-69,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划(2012BAI30B00);科技部科技人员服务企业行动项目(SQ2009GJG4101543);兵团科技支疆计划(2008ZJ14);石河子大学科学技术研究发展计划课题(ZRKX2009YB21)

摘　　要：目的:建立毛细管气相色谱法同时测定刺山柑果实中3种刺激性成分(异硫氰酸异丙酯、异硫氰酸甲酯、异硫氰酸异丁酯)的方法。方法:采用挥发油提取器提取挥发油;以异硫氰酸丁酯为内标物,用TM-FFAP毛细管柱分离,火焰离子化检测器检测;以保留时间定性,内标法定量。结果:在所选的色谱条件下,上述成分在46 min内获得较好的分离;3种成分与内标物的峰面积比值与其浓度呈良好的线性关系(r>0.999 1);样品加标平均回收率为85.9%～104%,RSD 2.44%～3.12%。结论:方法简单、准确,可同时测定刺山柑果实中3种刺激性成分的含量,为其质量控制提供依据。Objective： A capillary gas chromatographic method for the simultaneous determination of three irritant constituents （isopropyl isothiocyanate, methyl isothiocyanate, isobutyl isothiocyanate） in Cappers spinoza was developed. Method： The essential oil was extracted by steam distillation. Butyl isothiocyanate was selected as the internal standard substance. The contents of three irritant components was analyzed by gas chromatography using a TM-FFAP capillary column and a flame ionization detector. The retention time of the peak was applied for qualitative analysis, and the internal standard method was used for quantitative analysis. Result： Three components were well separated in 46 min. There were good linear relationships between peak area ratio on the mass concentration and correlation eoefficents above 0. 999 1. The average recoveries for the added standards and the RSD were 85.9%-104% and 2.44%-3.12%, respectively. Conclusion： This method is simple and accurate for the irritation determination of volatile substances of C. Spinoza.

关 键 词：气相色谱法 内标法 刺山柑果实 异硫氰酸异丙酯 异硫氰酸甲酯 异硫氰酸异丁酯 

分 类 号：R284.1[医药卫生—中药学]