导痰汤对颈动脉硬化家兔内皮细胞黏附分子-1及p21表达的影响  

Inhibition of Daotan Decoction on the Expression of ICAM-1 and p21 in Carotid Atherosclerosis Rabbits

作　　者：陈文强[1] 黄小波[1] 李宗信[1] 王芬[2] 王宁群[1] 

机构地区：[1]首都医科大学宣武医院,北京100053 [2]北京中医药大学附属东方医院,北京100078

出　　处：《中国实验方剂学杂志》2012年第16期154-157,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30772843;30801481;81102624);北京市科技新星项目(2008A90);北京市中医药科技项目(JJ2009-037)

摘　　要：目的:研究导痰汤是否能通过对内皮细胞黏附分子-1(intercellular adhesion molecular-1,ICAM-1)和p21通路的调节而干预颈动脉粥样硬化。方法:将25只雄性新西兰白兔随机分为正常对照组、模型组、导痰汤治疗组(分为高、中、低剂量组)。对照组给予基础饲料喂饲,模型组和导痰汤治疗组予以高脂饲料喂饲,并在喂饲1周后实施颈动脉内膜空气干燥术,术后导痰汤治疗组分别按照10,8,6 g.kg-1,ig每日给药1次。4周后处死家兔,取每只家兔右侧颈动脉远端1 cm,固定后行HE染色,观察病理改变。应用免疫组化SABC法检测ICAM-1和p21表达情况。结果:模型组血管内皮细胞、平滑肌细胞的胞浆中有ICAM-1(0.200±0.035)和p21(0.297±0.097)强阳性表达,显著高于对照组ICAM-1(0.037±0.005),p21(0.078±0.016),(P<0.01)。导痰汤低、中、高剂量治疗组细胞内ICAM-1(0.166±0.043,0.104±0.011,0.098±0.010)和p21(中剂量:0.173±0.021,高剂量:0.128±0.026)阳性表达显著低于模型组(P<0.05),且随剂量的增加,导痰汤对ICAM-1和p21的抑制逐渐增强;而p21与ICAM-1水平呈显著正相关(r=0.772,P<0.01)。结论:导痰汤可以有效抑制颈动脉硬化家兔血管内皮细胞中ICAM-1和p21的表达。Objective： To study the influence of Daotan decoction on the expression of intercellular adhesion molecular-1 （ICAM-1） and p21 in carotid atherosclerosis rabbits. Method： Twenty-five male rabbits were divided into control group, model group and Daotan decoction group. Control group was treated with basic diet; model group and Daotan decoction group were treated with both high-fat diet and carotid artery intima air drying operation. Daotan decoction group was treated with Daotan decoction, at dose of 10, 8, 6 mg ·kg^-1, for 4 weeks. Then the right carotid was cut and fixed. The expression of ICAM-1 and p21 was assayed by immunohistochemistry and pathological staining. Result： Compared with control group （ICAM-1 ： 0. 037 ± 0. 005, p21 ： 0. 078 ±0. 016）, the expression of ICAM-1 （0. 200±0. 035） and p21 （0. 297 ±0. 097） in model groupwas significantly increased （P 〈 0.01 ）. And compared with model group, Daotan decoction could significantly restrain ICAM-1 （low dose group： 0. 166±0. 043, medium dose group： 0. 104 ±0. 011, high dose group： 0. 098 ± 0. 010） and p21 （medium dose group： 0. 173± 0. 021, high dose group： 0. 128±0. 026） expression （P 〈 0.05）. Also, there was significant positive relationship between the expression of ICAM-1 and p21 （r = 0. 772, P 〈0.01）. Conclusion： Daotan decoction could restrain ICAM-1 and p21 expression in carotid atherosclerosis rabbits.

关 键 词：导痰汤 颈动脉硬化 细胞间黏附分子-1 P21 

分 类 号：R285.5[医药卫生—中药学]