复方青龙衣胶囊对人胃癌SGC-7901细胞凋亡的形态学影响  

Morphologic Influence of Compound Qinglongyi Capsules on Apoptosis in Human Gastric Cancer Cell SGC-7901

作　　者：张强[1] 段玉敏[1] 王伟明[1] 吴秉纯[1] 

机构地区：[1]黑龙江省中医研究院,哈尔滨150036

出　　处：《中国实验方剂学杂志》2012年第16期181-183,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大新药创制项目(2009ZX09102-138)

摘　　要：目的:研究复方青龙衣胶囊对人胃癌细胞SGC-7901细胞凋亡的形态学影响,探讨其抑制人胃癌细胞的作用机制。方法:复方青龙衣胶囊设定3个不同药物浓度梯度(终质量浓度分别为2.0×10-3,1.0×10-3,0.5×10-3g.L-1及阳性对照药羟基喜树碱(HCPT,0.5×10-3g.L-1)预处理人胃癌细胞SGC-7901 48 h,透射电镜观察其对SGC-7901细胞超微结构影响;荧光显微镜下观察其对SGC-7901细胞凋亡形态的影响。结果:以不同浓度的复方青龙衣处理人胃癌细胞SGC-7901 48 h,透射电镜结果显示,对SGC-7901细胞的超微结构有影响,提示可诱导细胞凋亡;荧光显微镜结果显示,经Hoechest33258染液染色后的SGC-7901细胞随着给药剂量的增大,凋亡细胞比例逐渐增加。结论:复方青龙衣胶囊可诱导人胃癌细胞SGC-7901细胞发生凋亡。Objective： To investigate the morphologic influence of compound Qinglongyi capsules on apoptosis as well as to preliminarily study the basis for its growth inhibitory effect on human gastric cancer cell. Method： Preprocessing human gastric cancer cell SGC-7901 for 48 hours by setting 3 different drug concentration gradients 2.0× 10^-3, 1.0 × 10^-3, 0.5× 10 ^-3 g · L^-1 observing its effect on ultrastructure of SGC-7901 cell by transmission electron microscope; observing its effect on apoptosis of SGC-7901 cell by fluorescence microscope. Result： After dealing with human gastric cancer cell SGC-7901 with compound Qinglongyi capsules of different concentration for 48 hours, the result of transmission electron microscope showed that the drughad effect on the ultrastructure of SGC-7901 cell and indicated that it can induce apoptosis. The result of fluorescence microscope showed that with the increase of the dose, the apoptosis of SGC-7901 ceils gradually increased in proportion after having been stained with Hoechest 33258 staining solution. Conclusion： Compound Qinglongyi capsule has obvious growth inhibitory effect on human gastric cancer cell SGC-7901, and it is probably able to induce apoptosis of cancer cells.

关 键 词：复方青龙衣胶囊 人胃癌SGC-7901细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]