抵挡汤早期干预对2型糖尿病大鼠大血管巨噬细胞调控作用的影响  

Effect of the Early Intervention with Didang Tang on Macrophages Regulation in Experimental Type 2 Diabetic Rats

作　　者：常柏[1] 李巧芬[2] 常宝成[1] 潘从清[1] 孟东[1] 陈莉明[1] 李春深[2] 

机构地区：[1]天津医科大学代谢病医院,天津300020 [2]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2012年第16期195-199,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973753)

摘　　要：目的:观察抵挡汤早期干预对实验性2型糖尿病大鼠大血管中巨噬细胞集落刺激因子(M-CSF)、巨噬细胞炎性蛋白(Mip-1a)和基质金属蛋白酶(MMP-9)表达的影响。方法:采用高脂饲料喂养及链脲佐菌素诱导法制成实验性大鼠糖尿病模型,分别以抵挡汤(含生药3.24 g.kg-1,ig)早期、中期及晚期干预,实时荧光定量PCR法检测主动脉M-CSF,Mip-1amRNA表达,Western-blot法检测主动脉MMP-9蛋白表达。结果:与模型对照组比较,抵挡汤早期干预组和罗格列酮组大鼠主动脉M-CSF mRNA表达明显降低(P<0.05);抵挡汤早期干预组、罗格列酮组和辛伐他汀组大鼠主动脉Mip-1a mRNA表达明显降低(P<0.05);抵挡汤早期、中期干预组和罗格列酮组MMP-9蛋白表达明显降低(P<0.05)。结论:抵挡汤早期干预可以有效降低实验性2型糖尿病大鼠主动脉M-CSF,Mip-1a mRNA表达以及MMP-9蛋白表达,调节巨噬细胞在动脉粥样硬化斑块形成及其稳定性中的作用,从而延缓糖尿病大血管病变的发展。Objective： To observe the effect of the early intervention with Didang Tang on the expressionlevels of macrophage colony-stimulating factor （M-CSF） , macrophage inflammatory protein-1 a （Mip-la） and matrix metalloproteinases-9 （MMP-9） associated with macrophages in the experimental type 2 diabetic rats with the macrovascular lesions. Method： The experimental diabetic in rats was induced by using high fat diet and streptozotocin, then early, middle and late intenventions of Didang Tang （3.24 g ·kg^-1, ig） were applied to the rats. The mRNA expression of M-CSF and Mip-la was detected by using Real-time PCR method, and the protein expression of MMP-9 was measured by using Western blot method in the aorta. Result： Compared with model group, the mRNA expression of M-CSF in the aorta were lower in the early Didang Tang group and rosiglitazone group （P 〈 0.05）. Compared with model group, the mRNA expression of Mip-la in the aorta was lower in the early Didang Tang group, rosiglitazone group and simvastatin group （P 〈 0.05）. Compared with model group, theprotein expression of MMP-9 in the aorta was lower in the early Didang Tang group, the middle Didang Tang group and rosiglitagone group （P 〈 0.05 ）. Conclusion： The mRNA expression of M-CSF and Mip-la and the protein expression of MMP-9 in the aorta of the experimental type 2 diabetic rats reduced after early intervened with Didang Tang. The early intervention with Didang Tang could regulate the role of macrophages in the formation and stability of atherosclerotic plaque, and delay the development of diabetic macroangiopathy.

关 键 词：抵挡汤 糖尿病 大血管病变 巨噬细胞集落刺激因子 巨噬细胞炎性蛋白.1 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学]