玉郎伞黄酮对四氯化碳诱导小鼠急性肝损伤的保护作用  

Protective Effect of Yulangsan Flavonoids on Acute Liver Injury Induced by Carbon Tetrachloride in Mice

作　　者：文坎[1] 郭又嘉[1] 蒙明瑜[1] 黄莎[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021

出　　处：《中国实验方剂学杂志》2012年第16期269-273,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科学研究与技术开发计划项目(桂科攻0630002-2A)

摘　　要：目的:研究玉郎伞黄酮(YLSF)对四氯化碳(CCl4)所致小鼠急性化学性肝损伤的保护作用及机制。方法:将小鼠分为正常对照组(NC),模型对照组(MC),YLSF高(YLSFH)、YLSF中(YLSFM)、YLSF低(YLSFL)剂量组,及联苯双酯组(BPDC)。NC组及MC组以生理盐水ig,各剂量YLSF组和阳性对照组分别给予YLSF(112,56,28 mg.kg-1)和BPDC(150 mg.kg-1)ig,连续给药7 d,每天1次。以分光光度法测定各组小鼠血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、碱性磷酸酶(ALP)活性、白蛋白(Alb)含量以及总抗氧化能力(T-AOC),以及肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性,并于光镜观察小鼠肝损伤程度。结果:YLSF高、中剂量YLSF能降低血清AST、ALT、ALP含量(P<0.01)及肝组织MDA含量(P<0.01),增加血清Alb含量(P<0.01),提升血清T-AOC水平(P<0.01)及肝组织T-SOD活力(P<0.01);低剂量YLSF能降低血清AST、ALP含量(P<0.01或P<0.05)及肝组织MDA含量(P<0.01),增加血清Alb含量,但对小鼠血清ALT含量、血清T-AOC水平及肝组织T-SOD活力的影响无统计学意义。同时,各剂量YLSF均能减轻肝细胞损伤程度(P<0.01,P<0.05)。结论:YLSF对CCl4诱导的小鼠急性化学性肝损伤具有显著保护作用,其作用机制可能与其抗氧自由基抑制脂质过氧化作用有关。Objective： To observe the protective effect of Yulangsan flavonoids （YLSF） on acute liver injury induced by carbon tetrachloride in mice. Method： The Kunming mice were divided into normal control group （NC） , model group （MC） , high-, medium-and low-doses of YLSF groups, and biphenyldicarboxylate group （BPDC）. The mice of NC and MC groups were treated with normal saline （NS）, and all doses of YLSF groups and the positive control group were treated with YLSF （112, 56, 28 mg · kg^-1） and BPDC （150 mg· kg^-1） respectively. All mice were treated with drugs or NS for 7 consecutive days ig, qd. One hour after the last administration of drugs, each group of mice was induced by 0. 1% CC14 , ip, except the NC group. The activities of aspartate aninotransfer as （AST）, alanine aminotransferase （ALT） and Alkaline phosphatase （ALP）, the content of Albumin （Alb）, the ability of Total antioxidant capacity （T-AOC） in serum, the content of Malondialdehyde （MDA）, and the activity of Superoxide dismutase （SOD） in liver tissue were investigated by spectrophotometry, and the degree of hepatic injury was examined. Result： In low-, medium-and high doses of the YLFS group, the activity of AST, ALT, Alb, ALP and the level of T-AOC in the serum were AST （40.49 ±10.41）, （32.97±2.58）, （27.57±7.98） U·L^-1, ALT （129.69±18.02）, （90.79±7.02）, （66.75±20.90） U·L^-1, Alb （4.53±1.05） , （5.15±1.62） , （5.68±0.72） g·L^-1 , ALP （40.06±1.47）, （35.77± 2.66）, （32.87±4.19） U·L^-1 and T-AOC （4.33±1.56）, （6.49±0.96）, （8.15±0.76） U·mL^-1, the activity of SOD and the content of MDA in the tissue were SOD （ 122.01±14.47）, （ 165.49 ± 18.25）, （ 191.66 ±37.95） U·mg^-1 and MDA （15.07 ±1.75）, （12.27±1.79）, （9.65±1.76） nmol ·mg^-1 respectively. The activities of AST, ALT and ALP in serum （P 〈 0.01） and the content of MDA in liver （P〈 0.01） were decreased, the content of Alb and the

关 键 词：玉郎伞 黄酮 四氯化碳 急性肝损伤 生化指标 自由基 

分 类 号：R285.5[医药卫生—中药学]