葛根芩连配方颗粒在大肠湿热证模型大鼠尿液中代谢产物研究  

Metabolites of Gegen Qinlian Dispensing Granule in urine of rats with large intestinal damp-heat syndrome

作　　者：陈丽红[1] 范慧佳[2] 唐于平[3] 卞勇[1] 杨念云[1] 井山林[1] 王强[4] 

机构地区：[1]南京中医药大学药学院,江苏南京210046 [2]合肥医工医药有限公司,安徽合肥230088 [3]南京中医药大学江苏省方剂研究重点实验室,江苏南京210046 [4]中国药科大学中药分析教研室,江苏南京210009

出　　处：《中草药》2012年第8期1570-1573,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30901995)

摘　　要：目的研究葛根芩连配方颗粒(简称葛根芩连颗粒)在大肠湿热证模型大鼠尿液中的代谢产物。方法采用综合因素(高糖和高脂饮食+高温和高湿环境+肠产毒性大肠杆菌致病因子)制备大肠湿热证大鼠模型;利用HPLC-DAD-MS技术对大鼠ig葛根芩连颗粒后尿液中代谢产物进行分析,检测波长272 nm;流动相A为0.1%甲酸水溶液,流动相B为乙腈-甲醇(6∶4),梯度洗脱。结果从给予葛根芩连颗粒的大鼠尿液中发现并鉴定了5种主要代谢产物,分别为葛根素、异甘草素、甘草素、汉黄芩素、千层纸素A,其中葛根素来自葛根,汉黄芩素与千层纸素A来自黄芩,异甘草素与甘草素来自甘草。结论在大肠湿热证模型大鼠尿液中,葛根芩连颗粒中黄芩与甘草的代谢产物主要以苷元为主,葛根中的葛根素主要以原形排出。Objective To study the metabolites of Gegen Qinlian Dispensing Granule （GQDG） in urine of rats model with large intestinal damp-heat syndrome. Methods The animal model with large intestine damp-heat syndrome was developed by multiple factors （high glucose and high fat diet, environment under high humidity and high temperature, and pathogenic biological factors of enterotoxigenic Escherichia coil）. The metabolites in urine of rats after ig administration of GQDG were analyzed by HPLC-DAD-MS. The detection wavelength was 272 nm; Mobile phase was 0.1% formic acid-water and acetonitrile-methanol （6：4）. Results Five components were identified as puerarin （from Puerariae Radix）, isoliquiritigenin, liquiritigenin （from Glycyrrhizae Preparata Radix）, wogonin, and oroxylin A （from Scutellariae Radix）. Conclusion Metabolites of Scutellariae Radix and Glycyrrhizae Preparata Radix in GQDG in urine of model rats with large intestinal damp-heat syndrome are mainly aglycone, and the metabolites of puerarin in Puerariae Radix are unchanged and mainly excreted by prototype via urine.

关 键 词：葛根芩连配方颗粒 大肠湿热证 代谢产物 葛根素 汉黄芩素 

分 类 号：R969.1[医药卫生—药理学]