采用两种细胞模型评价野菊花的寒热属性  

Evaluation on cold and hot characteristics of dried capitulum of Chrysanthemum indicum by two cellular models

作　　者：王征[1] 张宁[1] 成程[1] 李红民[1] 刘建利[1] 

机构地区：[1]西北大学生命科学学院西部资源与现代生物技术教育部重点实验室,陕西西安710069

出　　处：《中草药》2012年第8期1586-1589,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(20872118);教育部博士点基金资助项目(20070697012);陕西省重大科技专项资助项目(2008ZDKG-67);陕西省重点实验室基金资助项目(08JZ74;02JS15)

摘　　要：目的通过考察野菊花对人胃癌细胞株SGC-7901、人宫颈癌细胞株HeLa生长的促进或抑制作用,评价其寒热属性。方法用MTT比色法考察野菊花对HeLa细胞和SGC-7901细胞增殖的影响;倒置显微镜观察野菊花对细胞形态特征的影响;台盼蓝染色法分析野菊花对HeLa细胞和SGC-7901细胞的细胞毒活性。结果野菊花质量浓度≤20μg/mL时对细胞生长有促进作用,使细胞密度增加、结构致密、生长旺盛,单个细胞略变大。台盼蓝染色表明野菊花对两种细胞无细胞毒活性。结论野菊花药性温热,且对SGC-7901细胞、HeLa细胞无细胞毒活性。Objective To evaluate the cold and hot characteristic of the dried capitulum of Chrysanthemum indicum by two cellular models （growth promotion and inhibition of human cervical carcinoma HeLa and gastric carcinoma SGC-7901）. Methods MTT assay was used to investigate the effect of the dried capitulum of C. indicum on the growth of HeLa and SGC-7901 cells in vitro. Morphological changes of HeLa and SGC-7901 cells treated with the dried capitulum of C. indicum were observed through invert microscope. Trypan-blue staining was used to analyze the cytotoxicity of the dried capitulum of C. indicum on HeLa and SGC-7901 cells. Results The dried capitulum of C. indicum could promote the growth and proliferation of HeLa and SGC-7901 cells at the concentration〈~20 ~tg/mL. Morphological observation showed the dried capitulum of C. indicum could increase the cell density, structure density, and cell vitality, and single cell became bigger. Trypan-blue staining showed the dried capitulum of C. indicum had no cytotoxicity on HeLa and SGC-7901 cells. Conclusion The results reveal that the dried capitulum ofC. indicum shows a warm or hot characteristic and has no cytotoxicity.

关 键 词：野菊花 药性:HeLa细胞 SGC-7901细胞 细胞增殖 细胞毒活性 

分 类 号：R285.5[医药卫生—中药学]