长期人造二尖瓣植入绵羊模型的建立  

Establishment of a chronic model of mitral valve implantation in sheep

作　　者：王常田[1] 张利东[2] 张晓华[1] 钱雅君[1] 王军[1] 黄楠[3] 李德闽[1] 

机构地区：[1]南京军区南京总医院心胸外科,210002 [2]南京军区南京总医院麻醉科,210002 [3]西南交通大学材料学院

出　　处：《中华实验外科杂志》2012年第8期1456-1457,共2页Chinese Journal of Experimental Surgery

基　　金：国家重点基础研究973计划规划资助项目（G1999064705）

摘　　要：目的探讨建立长期存活的人造二尖瓣植入模型的方法。方法选用健康绵羊16只，雌雄各半，预实验6只，正式实验10只。麻醉成功后建立体外循环，经降主动脉插动脉灌注管，右心耳至右心房插静脉引流管，升主动脉根部置冷灌管，经左心耳显露二尖瓣，采用间断缝合方法植入人造二尖瓣。术后常规抗生素预防感染，口服华法林抗凝。结果正式实验中成功8例并长期成活达5个月以上，术后未出现并发症，实验结束行病理检查各脏器未见异常，2例死亡。结论绵羊作为长期存活人造二尖瓣植入模型的实验动物可行可靠、存活率高、术后易于管理。Objective To explore long-term survival of artificial mitral valve implantation model. Methods Healthy sheep （n = 16, male and female, pre-test 6, the formal experiment 10） were used to make models. Cardiopulmonary bypass was established after the success of anesthesia, artery infusion tube inserted in the descending aorta, venous drainage tube inserted through the right atrial appendage to fight atrial, cold irrigation tube inserted through aortic root. Mitral valve was exposed and removed through the left atrial appendage;artificial mitral valve was implanted by interrupted suture. Antibiotic was used to pre- vent infection, warfarin to anticoagulant conventionally after operation. Results Eight sheep survived to full term （5 months of more） in formal experiment. No postoperative complications were observed. Con- clusion This study demonstrated the feasibility and reliability of the sheep model for long-term survival of mirtal valve implantation. This sheep model provided improved rates of morbidity and mortality ease of post- operative care.

关 键 词：人造二尖瓣膜 绵羊 动物模型 

分 类 号：R654.2[医药卫生—外科学]