应用自体心包为材料制作肺动脉狭窄模型  

A novel material to create pulmonary artery stenosis in a swine model

作　　者：陈泽锐[1] 王德[1] 吴信[1] 潘湘斌[1] 洪亮[1] 李守军[1] 

机构地区：[1]北京协和医学院中国医学科学院阜外心血管病医院小儿心脏外科中心,100037

出　　处：《中华实验外科杂志》2012年第8期1458-1459,F0003,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨自体心包条带制作肺动脉狭窄动物模型的可行性。方法8头小型猪分别接受左侧第3肋间开胸，取自体心包条，用生理盐水浸泡30rain后，环缩左肺动脉至原始直径的30％～40％，术后1个月正中开胸，应用心导管及造影检查测量左肺动脉环缩术后跨狭窄段收缩压差及狭窄处肺动脉内径，行肺动脉支架置人术，3个月后取材行组织病理检查。结果左肺动脉环缩后即刻，左肺动脉内径从（16．0±0．4）mm减少到（6，4±0．2）mm（P〈0．01），而术中环缩后跨狭窄段压差为35（18～52）mmHg（中位数，极值，1mmHg=0．133kPa），环缩术后1个月与环缩后即时结果比较，动脉内径及跨狭窄段压差，差异均无统计学意义（P〉0．05）。环缩术后8只小型猪均存活,未出现环缩带松脱、断裂等并发症，也未出现左肺动脉发育不良或动脉闭锁。病理结果显示环缩处肺动脉未见钙化灶，周围肺动脉壁组织大致正常。结论在动物肺动脉狭窄模型制作中，应用自体心包环缩带是切实可行的。Objective To evaluate the effectiveness of autologous pericardium applied to pulmona- ry artery banding in a swine model. Methods In a swine model, pulmonary artery stenosis was created by autologous pericardium which made the left pulmonary artery banded to 30% -40% of its original diameter. Animals underwent right heart catheterization and angiography 4 weeks postoperatively, and balloon-ex- pandable＇stents were implanted through right ventricular outflow tract. The animals were sacrificed at 12th week following stenting and microscopic evaluation for histologic changes was made. Results Pulmonary artery banding was performed on 8 swines. The lumen diameter was decreased from （16. 0 ±0. 4） mm to （6.4 ±0. 2） mm （P 〈0. 01 ）, while the gradient increased from median （range） 0 mm Hg to 35 （18-52） mm Hg （ 1 mm Hg =0. 133 kPa,P 〈0.01 ） following banding. One month later, the models were all sur- vived. Compared to the instant result after banding, there was no significant difference in the lumen diame- ter （ P 〉 0. 05 ） or the gradient （ P 〉 0. 05 ） , and there was no banding dislodged and also no animal with severe left pulmonary artery hypoplasia or atresia. Conclusion Autologous pericardium as a banding to create an experimental pulmonary artery stenosis model appears safe and effective.

关 键 词：自体心包 肺动脉狭窄 

分 类 号：R654.3[医药卫生—外科学]