红茂草中异紫堇啡碱正交提取工艺的建立  

Extraction of isocorydine from Dicranostigma leptodum(Maxim.) Fedde by orthogonal design

作　　者：赵强[1,2] 王廷璞[3] 董晓宁[3] 沈彩霞[3] 赵海福[3] 

机构地区：[1]甘肃工业职业技术学院化工学院,甘肃天水741025 [2]甘肃农业大学动物医学院 [3]天水师范学院生命科学与化学学院

出　　处：《中兽医医药杂志》2012年第4期5-7,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省中医药管理局基金项目(GZK-2010-20)

摘　　要：采用正交工艺提取红茂草中异紫堇啡碱,在紫外光210 nm处对红茂草生物碱提取液进行检测,以异紫堇啡碱标准品作对照。结果正交提取最佳工艺参数为:乙醇浓度60%、乙醇用量200 mL、超声温度30℃、回流时间2.5 h。表明应用正交工艺对红茂草中异紫堇啡碱的提取比较合理。Orthogonal design was used to extract isocorydine from Dicranostigma Leptodum （Maxim.） Fedde, and the extracted liquid of alkaloids was detected by UV-VIS spectrophotometry at 210 nm using isocorydine as reference. The results demonstrated that the optimum conditions were ethanol concentration of 60%, alcohol consumption of 200 mL amt reflux time of 2.5 h at 30℃. The response surface analysis showed that this extraction process for isocorydine was reasonable.

关 键 词：红茂草 异紫堇啡碱 正交设计 

分 类 号：S853.74[农业科学—临床兽医学]