HPLC法测定温毒清浸膏中栀子苷的含量  

Content determination of geniposide in Wenduqing extractum by HPLC

作　　者：汤法银[1] 樊克锋[1] 王坤丽[1] 

机构地区：[1]郑州牧业工程高等专科学校药物工程系,河南郑州450008

出　　处：《中兽医医药杂志》2012年第4期51-53,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：河南省教育厅自然科学研究项目(2010A230013)

摘　　要：采用HPLC方法测定温毒清浸膏中栀子苷的含量。流动相:乙腈-水(15∶85);流速:1 mL/min;检测波长:238 nm;色谱柱:Diamonsi L C18(5μm,200 mm×4.6 mm);柱温:25℃;理论塔板数不低于1 500。结果显示,栀子苷进样量在0.060-0.600μg范围内有良好的线性关系,平均回收率99.32%,RSD为1.26%。表明该方法准确可靠,重现性好,可作为温毒清浸膏的质量控制方法。Objective： To determine the content of geniposide in Wenduqing extractum by HPLC. Methods： The mobile phase was acetonitrile and water （15：85）with the flow rate of 1 mL/min at 238 nm, the chromatographic column was Diamonsi L Cts （5 μm,200 min×4.6 mm） at 25℃, and theoretical plate number was no less than 1 500. Results： There was a good linearity for geniposide in Wenduqing extractum in the range of 0.060-0.600μg with average recovery rate of 99.32% and RSD of 1.26%. Conclusion： HPLC was accuracy and repeatability. suitable to use to control the quality of Wenduqing extractum for its accuracy and repeatability,

关 键 词：温毒清浸膏 栀子苷 含量测定 HPLC 

分 类 号：S853.74[农业科学—临床兽医学]