产帕曲星菌株的选育、抗菌物质纯化及结构鉴定  

Breeding of partricin prodcing strain,purification of antifungal metabolite and its structure verification

作　　者：刘海英[1,2] 陈代杰[2] 李继安[2] 邵雷[2] 蓝鸿[2] 

机构地区：[1]华东理工大学 生物工程学院,上海200237 [2]上海医药工业研究院 创新药物和制药工艺国家重点实验室,上海200040

出　　处：《工业微生物》2012年第4期7-12,共6页Industrial Microbiology

基　　金：国家863计划课题(863-2006Aa02z414);"重大新药创制"科技重大专项(2009ZX09301-007);上海市现场证物重点实验室开放课题(2011XCWZK09)

摘　　要：链霉菌SIPI-A.2020是本实验室分离保藏的一株可能产生帕曲星的放线菌,本文对此菌株培养与发酵、抗菌活性物质的分离纯化及其结构验证进行了研究。经过菌种选育与发酵条件优化,与出发菌株相比,主要发酵产物X的发酵效价提高89.3%;通过对发酵液的分离纯化,得到了HPLC纯度分别为96.3%和95.6%的两个组分,经进一步UV、MS、NMR等分析,验证其为帕曲星B和帕曲星A。Streptomyces SIPI-A. 2020 is an actinomycetes strain separated and preserved in our laboratory, which possibly producing partricin. In this paper, the culture and fermentation conditions, the separation and purification antifungal metabolites and the structure verification were discussed. By the ways of strain breeding and cultural conditio is of ns optimization, the fermentation titer of the main product X was increased by 89. 3% compared to that of the original strain. Two components were obtained through the separation and purification of fermentation broth, and the HPLC purity was 96.3% and 95.6% respectively. Further analyses by the methods of UV, MS, NMR, the two components separated were verified as partricin A and partricin B, respectively

关 键 词：SIPI—A.2020 帕曲星 结构验证 菌种与发酵 分离纯化 

分 类 号：TQ465[化学工程—制药化工]