天然放射性高本底地区居民凋亡基因bcl-2和bax表达水平研究  

mRNA expression level study on apoptosis-related genes bcl-2 and bax among residents in natural high background radiation area

作　　者：苏世标[1] 郭强之[1,2] 靳雅丽[3] 杨敏[1] 张丹英[1] 刘明[1] 陈建雄[1] 秦立强[4] 

机构地区：[1]广东省职业病防治院,广东广州510300 [2]中山大学公共卫生学院,广州510080 [3]广州市职业病防治院,510620 [4]苏州大学,215123

出　　处：《中国职业医学》2012年第4期279-281,共3页China Occupational Medicine

基　　金：苏州大学高校省级重点实验室开放课题(KJS1131)

摘　　要：目的探讨小剂量长期连续照射人群凋亡相关基因(抑制凋亡基因bcl-2和促凋亡基因bax)的mRNA表达水平。方法选择我国阳江天然放射性高本底辐射地区(HBRA)50名50～59岁男性居民为研究对象,另选择恩平市某镇(CA)出生并长大的年龄相仿的50名男性居民为对照人群。分别取其周围血10 ml并利用实时定量基因扩增荧光检测系统(QPCR)测定其白细胞bcl-2和bax的mRNA表达水平。结果在HBRA和CA人群中,bcl-2 mRNA相对表达量(RQ)分别为(1.26±0.59)、(1.03±0.39);bax mRNA RQ分别为(1.63±0.85)、(2.11±0.64);bcl-2 mRNA RQ/baxmRNA RQ分别为(0.92±0.10)、(0.54±0.05)。与CA组比较,HBRA组人群抑制凋亡基因bcl-2 mRNA RQ较高,促凋亡基因bax mRNA的RQ较低,指示抗凋亡能力的指标bcl-2 mRNA RQ/bax mRNA RQ值较高,以上指标在2组间的差异均有统计学意义(P<0.05或P<0.01)。结论小剂量长期连续电离辐射可能提高机体细胞存活基因的表达,降低促凋亡基因的表达。Objective To examine mRNA expression levels of apoptosis-related genes （ bcl-2 and bax） among residents under long-term low-dose continuous radiation exposure. Methods 50 residents aged from 50 to 59 in high background radiation area （HBRA） were selected as the exposure group and residents in Enping control area （CA） as the control group. 10 ml peripheral blood was drawn from the subjects, mRNA expression levels of genes bcl-2 and bax were determined with the method of Real-time Quantitative PCR Detecting System （QPCR）. Results The mRNA relative expression quantity （RQ） of genes bcl-2 and bax were （ 1.26 ± 0. 59 ） and （ 1.03 ±0. 39 ）, （ 1.63 ± 0. 85 ） and （2. 11 ± 0. 64） in HBRA and CA respectively. The ratios of bcl-2 mRNA and bax mRNA were （0. 92 ±0. 10） and （0. 54 ±0. 05） in HBRA and CA respectively. Compared with that of CA, the mRNA expression level of inhibitory apoptotic gene bcl-2 was higher in HBRA （ P 〈 0. 05 ）, the mRNA expression level of promotive apoptotic gene bax was lower in HBRA （P 〈0. 01 ） and the ratio of bcl-2 mRNA RQ and bax mRNA RQ was higher in HBRA （ P 〈 0. 01 ）. Conclusion Up-regulation of cell survival gene expression and down-regulation of apoptotic gene expression could be induced by long-term low-dose continuous radiation exposure.

关 键 词：高本底辐射 适应性反应 凋亡相关基因 BCL-2 BAX 

分 类 号：R714.43[医药卫生—妇产科学]