饮用水微生物的安全快速检测  

Fast and safety detection of drinking water in respect of microbial quality

作　　者：余辉[1] 马丽丽[1] 毛冠男[1] 王莹莹[1] 

机构地区：[1]南开大学环境科学与工程学院环境污染过程与基准教育部重点实验室天津市城市生态环境修复与污染防治重点实验室,天津300071

出　　处：《微生物学通报》2012年第8期1171-1178,共8页Microbiology China

基　　金：国家自然科学基金项目(No.31000247);高等学校博士学科点专项科研基金课题(No.20100031120018)

摘　　要：【目的】为了更好地分析饮用水中的微生物含量。【方法】利用流式细胞术(Flowcytometry,FCM)、ATP测定方法检测瓶装无气饮用水中的微生物数量、可同化有机碳(Assimilable organic carbon,AOC)含量以及微生物活性,并将检测结果与传统的饮用水微生物检测技术相对照。【结果】FCM方法可快速区分水样中的活性细菌和非活性细菌,AOC含量反映了水样中微生物再生能力;而ATP检测方法也能比异养细菌平板计数法(Heterotrophic plate count,HPC)更好地反映瓶装无气饮用水中的实际微生物含量。【结论】FCM、ATP测定方法要明显优于依赖于培养的传统方法。[Objective] The current study investigates the microbial quality of drinking water using various microbial detection methods. [Methods] We analyzed the microbial quality of still bottled drinking water using flow cytometric cell count, ATP assay, AOC （assimilable or-ganic carbon） assay and cell viability test. [Results] We found that the flow cytometry allowed fast and accurate discrimination of the live/dead bacteria and detection of the micro-scale AOC. Our results also showed that ATP is a better indicator than the heterotrophic plate counts of the actual active microorganism content in still bottled drinking water. [Conelusion] The results suggested that the flow cytometric cell count and ATP assay are more suitable methods for assessing microbial quality in bottled drinking water than the conventional heterotrophic plate count method.

关 键 词：饮用水 流式细胞术 微生物活性 可同化有机碳 细菌再生长能力 

分 类 号：R123.1[医药卫生—环境卫生学]