A Study of Interaction between Flavonoids and the Parallel Quadruplex Structure [d（TGGGGT）]4 by Electrospray Ionization Mass Spectrometry  

作　　者：徐牛生 杨洪梅 崔勐 宋凤瑞 刘志强 刘淑莹 

机构地区：[1]Changchun Center of Mass Spectrometry,Changehun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,Jilin 130022,China [2]Graduate School of the Chinese Academy of Sciences,Beijing 100039,China [3]Changchun University of Chinese Medicine,Changchun,Jilin 130117,China

出　　处：《Chinese Journal of Chemistry》2012年第7期1433-1438,共6页中国化学（英文版）

基　　金：Acknowledgement This work was supported by the National Natural Science Foundation of China （No. 28073137）.

摘　　要：In this study, electrospray ionization mass spectrometry （ESI-MS） was used to investigate interaction of 21 flavonoids （10 aglycones and 11 glycosides） with the parallel quadruplex structure [d（TGGGGT）]4. Relative binding affinities of flavonoids toward [d（TGGGGT）]4 were estimated based on the fraction of bound DNA. It was found that [d（TGGGGT）]4 showed a binding preference to the flavonoid glycosides over flavonoid aglycones. It was d duced that glycosylation played a key role for the [d（TGGGGT）]a-binding properties of flavonoid glycosides. Upon collision-induced dissociation, complexes of flavonoid/[d（TGGGGT）]4 underwent the loss of flavonoids, suggesting an end-stacking binding mode. The current work demonstrates that ESI-MS is a powerful tool in the study of irheraction between drugs and nucleic acids.In this study, electrospray ionization mass spectrometry （ESI-MS） was used to investigate interaction of 21 flavonoids （10 aglycones and 11 glycosides） with the parallel quadruplex structure [d（TGGGGT）]4. Relative binding affinities of flavonoids toward [d（TGGGGT）]4 were estimated based on the fraction of bound DNA. It was found that [d（TGGGGT）]4 showed a binding preference to the flavonoid glycosides over flavonoid aglycones. It was d duced that glycosylation played a key role for the [d（TGGGGT）]a-binding properties of flavonoid glycosides. Upon collision-induced dissociation, complexes of flavonoid/[d（TGGGGT）]4 underwent the loss of flavonoids, suggesting an end-stacking binding mode. The current work demonstrates that ESI-MS is a powerful tool in the study of irheraction between drugs and nucleic acids.

关 键 词：FLAVONOIDS G-quadruplex DNA INTERACTION ESI-MS 

分 类 号：O631.12[理学—高分子化学] Q946.8[理学—化学]