Immobilization and Bioelectrochemistry of Hemoglobin Based on Carrageenan and Room Temperature Ionic Liquid Composite Film  

作　　者：盛春 张洋 王璐 贾能勤 

机构地区：[1]The Education Ministry Key Laboratory of Resource Chemistry,Department of Chemistry,College of Life and Environmental Sciences,Shanghai Normal University,100 Guilin Road,Shanghai 200234,China

出　　处：《Chinese Journal of Chemistry》2012年第7期1565-1570,共6页中国化学（英文版）

摘　　要：A novel biopolymer/room-temperature ionic liquid composite film based on carrageenan, room temperature ionic liquid （IL） [1-butyl-3-methylimidazolium tetrafluoroborate （[BMIM]BF4）] was explored for immobilization of hemoglobin （Hb） and construction of biosensor. Direct electrochemistry and electrocatalytic behaviors of Hb entrapped in the IL-carrageenan composite film on the surface of glassy carbon electrode （GCE） were investigated. UV-vis spectroscopy demonstrated that Hb in the IL-carrageenan composite film could retain its native secondary structure. A pair of well-defined redox peaks of Hb was obtained at the Hb-IL-carrageenan composite film modified electrode through direct electron transfer between the protein and the underlying electrode. The heterogeneous electron transfer rate constant （ks） was 2.02 s 1, indicating great facilitation of the electron transfer between Hb and IL-carrageenan composite film modified electrode. The modified electrode showed excellent electrocatalytic activity toward reduction of hydrogen peroxide with a linear range of 5.0 × 10-6 to 1.5 ×10-4 mol/L and the detection limit was 2.12 ×10 7 mol/L （S/N= 3）. The apparent Michaelis-Menten constant KM^app for hydrogen peroxide was estimated to be 0.02 mmol/L, indicating that the biosensor possessed high affinity to hydrogen peroxide. In addition, the proposed biosensor showed good reproducibility and stability.A novel biopolymer/room-temperature ionic liquid composite film based on carrageenan, room temperature ionic liquid （IL） [1-butyl-3-methylimidazolium tetrafluoroborate （[BMIM]BF4）] was explored for immobilization of hemoglobin （Hb） and construction of biosensor. Direct electrochemistry and electrocatalytic behaviors of Hb entrapped in the IL-carrageenan composite film on the surface of glassy carbon electrode （GCE） were investigated. UV-vis spectroscopy demonstrated that Hb in the IL-carrageenan composite film could retain its native secondary structure. A pair of well-defined redox peaks of Hb was obtained at the Hb-IL-carrageenan composite film modified electrode through direct electron transfer between the protein and the underlying electrode. The heterogeneous electron transfer rate constant （ks） was 2.02 s 1, indicating great facilitation of the electron transfer between Hb and IL-carrageenan composite film modified electrode. The modified electrode showed excellent electrocatalytic activity toward reduction of hydrogen peroxide with a linear range of 5.0 × 10-6 to 1.5 ×10-4 mol/L and the detection limit was 2.12 ×10 7 mol/L （S/N= 3）. The apparent Michaelis-Menten constant KM^app for hydrogen peroxide was estimated to be 0.02 mmol/L, indicating that the biosensor possessed high affinity to hydrogen peroxide. In addition, the proposed biosensor showed good reproducibility and stability.

关 键 词：CARRAGEENAN room temperature ionic liquid HEMOGLOBIN direct electrochemistry 

分 类 号：TQ658.41[化学工程—精细化工] O645.4[理学—物理化学]