Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross-linking  

作　　者：刘玉革 李伟明 魏长宾 吕玲玲 

机构地区：[1]South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Science,Zhanjiang,Guangdong 524091,China

出　　处：《Chinese Journal of Chemistry》2012年第7期1601-1604,共4页中国化学（英文版）

基　　金：Acknowledgement The work was supported by the Fund on Basic Scientific Research Project of Nonprofit Central Research Institutions （No. SSCRI200901） and the Natural Science Foundation of Hainan Province （No. 310099）.

摘　　要：Here in this paper, xanthine oxidase （XOD） was immobilized onto the chitosan （CHT） modified electrode by a simple way of cross-linking with glutaraldehyde （GTD） and 3-aminopropyltriethoxysilane （KH）. The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 μmol/L with a low detection limit of 0.0215 μmol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxantbine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.Here in this paper, xanthine oxidase （XOD） was immobilized onto the chitosan （CHT） modified electrode by a simple way of cross-linking with glutaraldehyde （GTD） and 3-aminopropyltriethoxysilane （KH）. The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 μmol/L with a low detection limit of 0.0215 μmol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxantbine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.

关 键 词：SENSOR xanthine oxidase CHITOSAN cross-linking 

分 类 号：TQ264.12[化学工程—有机化工] TS252.1[轻工技术与工程—农产品加工及贮藏工程]