无标记金纳米传感测定四氯苯醌  

Label-free colorimetric detection of tetrachloro-p-benzoquinone with Au nanoparticles

作　　者：姚英华 李程程[2] 蔡青云[2] 

机构地区：[1]湖南益阳龙州中学。湖南益阳431000 [2]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《化学传感器》2012年第2期35-39,共5页Chemical Sensors

基　　金：国家973项目支持

摘　　要：活体内五氯苯酚(PCP)的检测,往往是通过检测其代谢产物实现。四氯苯醌(TCBQ)作为PCP的一种主要代谢产物,发展其检测方法具有重要意义。该文基于TCBQ与ssDNA的G碱基发生特异性化学反应,破坏ssDNA的结构,从而破坏ssDNA对金纳米粒子的保护作用,导致金纳米粒子在盐诱导下聚沉发生颜色改变。通过测定ssDNA保护的金纳米粒子溶液颜色的改变,实现TCBQ的定量检测。该检测方法线性范围宽(9.3nmol/L～0.93mmol/L),检测限达到了3nmol/L。干扰实验表明该方法选择性高。The detection of PCP in vivo is generally realized through the detection of tetrachloro-p-benzoquinone （TCBQ）, a metabolite of PCP. As one of the major metabolite of PCP, it is of great interests to develop its detection techinque. In this work, a label-free colorimetric detection of TCBQ is developed based on the specific reaction between TCBQ and G bases of ssDNA. The TCBQ-ssDNA reaction changes the structure of ssDNA, leading to the destroy of the ssDNA-protected gold nanoparticles （NPs）, and cosequently the salt-induced aggregation of Au NPs. TCBQ was detected based on the TCBQ-ssDNA reaction-resulted change of the Au NPs solution. The proposed method exhibits a wide linear range of 9.3 nmol/L -0.93 mmol/L, and a high sensitivity with a limit of detection （LOD） of 3 nmol/L. Interference experiments demonstrate this method is with a good selectivity.

关 键 词：纳米金 探针 五氯酚 四氯苯醌 持久性有机污染物 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]