山葡萄雄株花蕾性别转换的形态观察及其内源激素的变化  

Morphological and Endogenous Hormones Changes on Sex Conversion of Male Vitis amurensis

作　　者：焦竹青[1] 王振兴[1] 许培磊[1] 艾军[1] 

机构地区：[1]中国农业科学院特产研究所,吉林长春130112

出　　处：《北方园艺》2012年第16期5-8,共4页Northern Horticulture

基　　金：现代农业产业技术体系建设专项资金资助项目(nycytx-30-01);农业部保种资助项目(NB2011-2130135-37);国家农作物种质资源平台运行服务资助项目

摘　　要：以山葡萄雄株为试材,花前20d用100mg/L的KT-30S处理雄株花序,以蒸馏水处理为对照,观察处理后的形态变化和坐果情况,并在不同处理时期取样测定内源激素含量,探讨外源细胞分裂素KT-30S对山葡萄雄株花蕾性别转换的效果及其对内源激素的影响。结果表明:KT-30S对山葡萄雄株的性反转效果显著,雄花花蕾在处理6d子房显著膨大。经KT-30S处理,4种内源激素含量和平衡关系表现出不同的变化趋势,由此推断,KT-30S诱导山葡萄雄株性反转可能是通过调节内源激素在不同时期的水平及其之间的平衡关系来实现的。The flower clusters of male vines were treated with KT-30S about 20 days before anthesis,those treated with water as control.The flower buds morphology were observed and content of four endogenous hormones（abscisic acid（ABA）,gibberellic acid（GA）,indole-3-acetic acid（IAA）,zeatin riboside（ZR）） were measured in both treatment and control flower buds at different treatment time.The flower buds of male Vitis amurensis cultivar were treated with exogenous cytokinin KT-30S to relize the changes of flower morphology and endogenous hormones involved in sex conversion of flower buds.The results showed that KT-30S was effect in sex conversion of male Vitis amurensis.The flower buds of male Vitis amurensis altered sex from male to hermaphrodite at 6 days after treatment.The content and the ratios of endogenous hormones in treatment flower buds performed differently compare to that in control flower buds at different stages of sex conversion.These results suggested that quantitative changes and the balance between endogenous hormones at different stages were involved in the control of sexuality in flower buds of male Vitis amurensis.

关 键 词：山葡萄雄株 花蕾 细胞分裂素KT-30S 性别转换 内源激素 

分 类 号：S663.1[农业科学—果树学]