何首乌提取物抗抑郁活性研究  

Antidepression activity of extract of Heshouwu(Radix Polygoni Multiflori)

作　　者：王君明[1] 朱新瑞[1] 

机构地区：[1]河南中医学院药学院,河南450008

出　　处：《北京中医药大学学报》2012年第7期449-451,455,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30772794);河南中医学院博士科研基金项目(No.03301092)

摘　　要：目的首次考查何首乌提取物抗抑郁活性。方法采用昆明KM雄性小鼠,每组10只。何首乌水提物(HW)、何首乌75%乙醇提取物(HE)及阳性药盐酸氟西汀(FH)均以0.5%羧甲基纤维素纳(CMC-Na)溶解或混悬至所需给药浓度,HW和HE剂量均分别为1.5、3 g/kg,FH剂量为10mg/kg,模型组给予同等体积的0.5%CMC-Na,各组均连续灌胃给药7 d,每天1次,末次给药1 h后,开展小鼠悬尾实验(TST)、强迫游泳实验(FST)和开场实验(OFT)测试。结果 HW(1.5、3 g/kg)可使小鼠在TST及FST的不动时间均显著性缩短(P<0.05),呈现出良好的量效关系,且与阳性药FH相比无显著性差异(P>0.05),而HE(1.5、3 g/kg)对小鼠在TST及FST的不动时间均无显著性差异(P>0.05);各组小鼠OFT穿格次数比较,均无显著性差异(P>0.05)。结论首次证实何首乌水提物具有抗抑郁活性,而其醇提物在既定给药方案下无抗抑郁活性。Objective To examine for the first time the antidepression activity of extract of Heshouwu （Radix Polygoni Multiflori）. Methods Male Kunming mice were divided into groups （each n = 10）. The aqueous extract and ethanol （75 % ） extract of Heshouwu and fluoxetine hydrochloride （FH） were all dissolved or suspended in 0.5% CMC-Na to reach to desired concentration. The doses of aqueous extract were 1.5 g/kg and 3 g/kg respectively, and ethanol extract, also 1.5 g/kg and 3 g/kg respectively, and dose of FH was 10 mg/kg. Equivalent dose of 0.5% CMC-Na was given to model group. All groups were intragastrically given corresponding medicinals once a day for 7 days. After the last time of giving medicinals for one hour, the tail suspension test（ TST）, forced swimming test（FST） and open field test（OFT） were conducted in mice. Results The aqueous extract （ 1.5 g/kg and 3 g/kg） shortened significantly the dead time in TST and FST （ P 〈 0.05 ） showing a good dose-effect relation. There was no significant difference compared with FH（P 〉 0.05 ）. The ethanol extract（ 1.5 g/kg and 3 g/kg） had no significant differences in the changes of the dead time in TST and FST（ P 〉 0.05 ）. The comparison of crossing times in OFF was not different among all groups （ P 〉 0.05 ）. Conclusion It is proved for the first time that the aqueous extract of Heshouwu has antidepression activity, while its ethanol extract had no under an established medicinal administration plan.

关 键 词：抗抑郁 何首乌 水提物 醇提物 悬尾实验 强迫游泳实验 开场实验 

分 类 号：R285.5[医药卫生—中药学]