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作 者:傅巧娟[1] 沈国正[1] 李春楠[1,2] 崔海瑞[2]
机构地区:[1]杭州市农业科学研究院园艺研究所,浙江杭州310024 [2]浙江大学原子核农业科学研究所,浙江杭州310029
出 处:《核农学报》2012年第4期656-659,共4页Journal of Nuclear Agricultural Sciences
基 金:浙江省科技计划项目(2004C22014);农业部公益性行业科研专项经费(201103007)
摘 要:用一串红品种帝王自交系种子搭载"神舟4号"宇宙飞船,进行空间诱变处理,诱变后代材料经多代选择,育成了新品种神州红,2008年12月通过浙江省非主要农作物品种委员会认定。与原品种帝王相比,神州红的开花期、单花序观赏期和成活率明显改善,但其他性状差异不显著。SRAP分析结果显示,在所用的33对SRAP引物组合中有17对呈现多态性,二者的遗传相似系数为0.945,说明空间诱变处理引起了一串红遗传物质的变异。本研究的结果证明,空间诱变对一串红是一种有效的育种途径,SRAP技术是分析空间诱变的有效方法。A new scarlet sage(Salvia splendens) variety Shenzhouhong was obtained by several generation selections from the progeny of the variety Diwang carried by the spacecraft Shenzhou No. 4 for space mutagenesis. Shenzhouhong was approved to release by Non-major Crop Identification Committee of Zhejiang Province in December 2008. Compared with its original variety Diwang, Shenzhouhong was significantly improved in survival rate, flowering time and ornamental period of single flower branch. SRAP (sequence-related amplified polymorphism) analysis showed that 17 of 33 pairs of primer combinations tested in total were polymorphic and the genetic similarity coefficient between them was 0. 945, suggesting that the genetic variation was happened after space mutagenesis. Our results proved that space mutagenesis was a kind of effective breeding approach for Salvia splendens and the SRAP was an efficient method for analysis of space-induced mutations.
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