内含子增强脂质体介导的转基因表达  

Enhancement of intron on gene expression after liposome-mediated transfection

作　　者：王泽平[1] 张景迎[1] 庞呈义[1] 

机构地区：[1]泰山医学院生物化学与分子生物学教研室,山东泰安271000

出　　处：《泰山医学院学报》2000年第2期92-94,共3页Journal of Taishan Medical College

摘　　要：:目的　观察内含子对脂质体介导的转基因表达的影响。方法　首先构建含内含子intronA、IL 2基因、IFN γ基因的质粒表达载体 ,然后经阳离子脂质体LipofectAMINE介导转染小鼠成纤维细胞NIH3T3。生物活性法检测IL 2基因或IFN γ基因表达水平。结果　含内含子的载体其IL 2或IFN γ表达水平高于不含内含子的载体(P <0 0 5 )。Objective: To observe the effect of intron on gene expression after liposome mediated transfection. Methods: First, the plasmid expression vectors carrying intron A, IL 2 gene , IFN γ gene, p53 were constructed. And then, these genes were transfected into NIH3T3 cells with LipofectAMINE. The expression level of IL 2 and IFN γ in culture supernatant was examined by means of biological activity. Results: Higher gene expression level of IL 2 or IFN γ in intron carrying vectors was observed as compared with non intron vectors. Conclusion: The gene expression level may be enhanced by intron A after liposome mediated transfection.

关 键 词：内含子 脂质体 白细胞介素2 Γ-干扰素 基因表达 

分 类 号：R456[医药卫生—治疗学]